Protein Production Service
Escherichia coli (E. coli) provides for a relatively simple, fast, inexpensive and robust system for native and heterologous recombinant protein production. While unable to render many of the complex eukaryotic post-translational modifications, technologies such as co-expression of chaperones/kinases/rare tRNAs and engineering of strains that provide a pro-disulfide bond (oxidative) environment hallmark the technological progress achieved in the E. coli system over the last two decades. abm recommends the E. coli system when post-translational modifications such as glycosylation are not crucial to the intended downstream application(s).
The mammalian expression system provides the only authentic and feasible option in cases where the protein of interest needs to be embellished with characteristic, mammalian post-translational modification(s) and/or entails a complex translational machinery for proper folding and targeting (such as membrane proteins). Therefore, abm recommends its mammalian expression service for proteins for which the native folding and post-translational modifications are of a complex nature (beyond the purview of E. coli) and indispensable to the customer's intended application(s).
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Bacterial Expression
Mammalian Expression
"I am a fan of abm: great product quality, great price, great rewards and great experience."
Dr. Vincent Emond, Centre de recherche du CHU de Québec CHULService Details
Custom protein production in E. coli| Services | Cat. No. | Unit | Price |
|---|---|---|---|
|
C103 | 1 Service | Inquire |
| Add-On Services | |||
| Special Buffer Request | C118 | 1 Service | Inquire |
| Special Concentration Request | C119 | 1 Service | Inquire |
| Lyophilization Request | C125 | 1 Service | Inquire |
| Endotoxin testing and removal (<1EU/µg of protein) | C194 | 1 Service | Inquire |
Custom protein production in Mammalian cells
| Services | Cat. No. | Unit | Price |
|---|---|---|---|
|
C113 | 1 Service | Inquire |
| Add-On Services | |||
| Special Buffer Request | C118 | 1 Service | Inquire |
| Special Concentration Request | C119 | 1 Service | Inquire |
| Lyophilization Request | C125 | 1 Service | Inquire |
Note:
- A 30% non-refundable deposit is required to initiate production
Additional Info
Disclaimer for Custom protein production in E. coli
- Disclaimer for intended use: All of abm's recombinant proteins are for research use only and not intended for therapeutic/diagnostic applications. abm is not liable for any violation (and associated repercussions) to the specified use of its entire suite of recombinant proteins.
- Disclaimer for 'E. coli' expression system: The choice of utilizing a prokaryotic expression system (Escherichia coli) for eukaryotic protein production is solely up to the discretion of the customer with respect to being aware of the limitations to the rendition of correct number and type of Post Translational Modifications (PTMs).
- Disclaimer for extra amino acids: abm does not guarantee that the recombinant protein product will be free of any additional amino acids residues. Cloning may lead to the insertion of some amino acids at either and/or the N and C-terminus of the recombinant protein product which in most cases, is innocuous to the stability/functionality of the recombinant protein.
- Disclaimer for turnaround time: Note that the turnaround time indicated above is our best estimate currently. Final turnaround time is subject to change depending on actual experimental conditions.
- Disclaimer for functionality: abm is not in a position to guarantee protein functionality.
Disclaimer for Custom protein production in Mammalian cells
- Disclaimer for intended use: All of abm's recombinant proteins are for research use only and not intended for therapeutic/diagnostic applications. abm is not liable for any violation (and associated repercussions) to the specified use of its entire suite of recombinant proteins.
- Disclaimer for extra amino acids: abm does not guarantee that the recombinant protein product will be free of any additional amino acids residues. Cloning may lead to the insertion of some amino acids at either and/or the N and C-terminus of the recombinant protein product which, in most cases, is innocuous to the stability/functionality of the recombinant protein.
- Disclaimer for turnaround time: Note that the turnaround time indicated above is our best estimate currently. Final turnaround time subject to change depending on actual experimental conditions.
- Disclaimer for functionality: Note that abm is not in a position to guarantee downstream functionalities.
- By default, a His tag will be used for purification purposes. Otherwise, additional charges apply and please provide a verified gene expression antibody along with a positive control.
- Charges for C112 and C113 determined after customer has reviewed report from C111 and requested for Phase II. An estimate can be provided during quotation process.
Related Products
Documents
FAQs
| For E. coli protein expression, is the purified protein delivered denatured or folded? |
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The protein will be purified without any denaturing steps but we cannot guarantee whether the protein is folded properly within the E. coli system.
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