Custom CRISPR sgRNA Non-Viral Vectors
abm offers Custom All-in-One (Cas9 and sgRNA expressing) and sgRNA only expressing Non-Viral Vectors that will simplify and streamline your CRISPR/Cas9 experiment. Choose from our All-in-One system or our sgRNA only expression system and pair the latter with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease. Just tell us what you want to accomplish and we'll do the sgRNA design and cloning for you.
A non-viral vector from abm is the simplest approach to deliver sgRNAs into cells, ideal for expressing sgRNA in a transient manner to limit off-target effects. When the non-viral vector has done its work, it can be diluted out of the cell population by passaging; just monitor the GFP fluorescence signal until it is gone.
Available Custom CRISPR Vectors and Viruses:
| AAV Vector /Virus |
Lentivector /Virus |
Non-Viral Vector |
Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| Browse Collection | Browse Collection | Browse Collection | Browse Collection |
Testimonial:
"Construct works beautifully, definitely will get more if needed."
Emily Chen, UCLA, CRISPR ConstructsAdditional Resources:
Service Details
| Service Name | Vector | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – single target | pNV-sgRNA-GFP | 1.0 ug | C439 | $135.00 |
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – set of three targets | pNV-sgRNA-GFP | 3 x 1.0 ug | C443 | $360.00 |
| Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – single target | pNV-sgRNA-Cas9-2A-GFP | 1.0 ug | C440 | $125.00 |
| Custom CRISPR All-in-One Non-Viral Vector(with spCas9) – set of three targets | pNV-sgRNA-Cas9-2A-GFP | 3 x 1.0 ug | C444 | $355.00 |
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Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359 |