Fine-Tuning Gene Expression Vectors (Custom miR-Tuners)
Conventional mammalian expression systems rely on the usage of strong viral promoters (i.e. CMV and SV40) which over-express the gene of interest (GOI). However, over-expression of a foreign gene can lead to detrimental effects in the cell and importantly, fails to replicate normal physiological gene expression levels. To address this issue, miR-Tuner Vectors can be used to precisely fine-tune transgene expression levels in a predictable manner.
miR-Tuners are short, synthetic miRNA response element sequences which reside in the 3’ UTR of the GOI. These sequences are responsive to a native miRNA species which is widely expressed in many human and mouse cells. Using the miR-Tuner Low, Med or Hi will allow for precise low, medium or high expression levels of the GOI, respectively.
abm offers a variety of miR-Tuner Vector products which can precisely and predictably control transgene expression levels in mammalian cells. These products include ready-to-clone miR-Tuner MCS Lentiviral Vectors and Custom Cloning Services of your GOI into a set of miR-Tuner Vectors/Lentiviruses.
Applications
Fine-tuning transgene expression levels in mammalian cells for:
- Engineered genetic circuits and cell lines
- Studying physiological and biochemical changes
Additional Resources:
"Always amazed at the reasonable prices and breadth of selection for genes of interest available on your site. Thanks very much!"
Brian DeBosch, Washington University in St. LouisService Details
| Service | Unit | Cat. No. | Price |
|---|---|---|---|
| 1. Choose Insert Format | |||
| a. ORF (choose from our existing ORF Library) | Not a Deliverable | N/A | From $45.00 |
| b. Gene Synthesis | Per bp | C098 | Starting from USD $0.18/bp |
| 2. Choose Product Format | |||
| a. Vector Options: | |||
| miR-Tuners Set of 3 custom cloned vectors (puromycin) | 3 x 10 µg | LV106 | $895.00 |
| miR-Tuners Set of 3 custom cloned vectors (NGFR) | 3 x 10 µg | LV107 | $995.00 |
| b. Lentivirus Options: | |||
| miR-Tuners Set of 3 lentivirus (puromycin), 107 IU/ml | 2 x 200 µl each virus | LV108 | $1850.00 |
| miR-Tuners Set of 3 lentivirus (NGFR), 107 IU/ml | 2 x 200 µl each virus | LV109 | $1950.00 |
Controls
| Product | Unit | CAT. NO. | Price |
|---|---|---|---|
| miR-Tuners Set of 3 Control vectors LV104-Low LV104-Med LV104-Hi |
3 x 10 µg | LV104 | $350.00 |
| miR-Tuners Set of 3 Control Lentiviruses, 107 IU/ml LV105-Low LV105-Med LV105-Hi |
3 x 200 µl | LV105 | $850.00 |
Supporting Data
The reporter gene Firefly Luciferase was cloned into the set of miR-Tuner Lentivectors and transfected into HEK293T cells. Following 24h incubation, the cells were lysed and subjected to a Luciferase Assay with the output RLU measured on a standard plate reader. As seen below, miR-Tuner Vectors were able to predictably control Firefly Luciferase gene expression.
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