Gene Expression Assay Service
abm’s Gene Expression Assay Service utilizes RT-PCR technology, with gene-specific primers designed to provide an expression profile for your relevant target genes. Each service includes primer & cDNA synthesis, PCR assay setup, and data analysis. Samples can be submitted as either:
- Total RNA shipped on dry ice, or
- Cell pellets shipped on dry ice (Custom RNA Isolation Service will apply per sample; Cat# C207, $450)
A certificate of analysis will be provided with each service.
Additional Resources:
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Dr. Vincent Emond, Centre de recherche du CHU de Québec CHULService Details
| Service | Unit | CAT. NO. | PRICE |
|---|---|---|---|
| Gene Expression Assay Service^ | 1 Marker | C138 | $165.00 |
^A Custom RNA Isolation Service will apply (Cat# C207, $450); up to 4 markers can be tested per RNA extraction service.
If the gene of interest is a common cell type marker (listed above), abm can design the primers free of charge.
Additional Info
Common Cell Type Markers (free primer design service included)
| Cell Type | Marker |
|---|---|
| Epithelial | CK18, CK19, Ep-CAM |
| Endothelial | vWF, VE-Cadherin |
| Smooth Muscle | SMα-actin |
| Fibroblast | Vimentin |
Sample Submission Guideline
| Total RNA | Minimum 2 ug / marker |
| Cell Pellets | Minimum 1 million cells / marker |
Related Products
Coming SoonDocuments
FAQs
| Do I have to design or send the primers to detect my marker of interest? |
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If the gene of interest is a common cell type marker (listed above), then we can design the primers free of charge. If there is a specific gene of interest to be detected, we would prefer that the customer sends us the sequences of validated primers. Customers do not need to send in the physical primers, but simply provide their sequence(s). If we need to design specific primers as part of the service, additional charges may apply and the lead time will be extended.
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| If I am providing primer designs, how many primer designs can I provide? |
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Customers can provide a maximum of 2 primer designs per marker. If you would like us to test additional primers, additional charges will apply.
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