abm may already offer a cDNA adenovirus for your gene of interest in Human, Mouse, or Rat. However, if you want to express a specific isoform, or express siRNA/RNAi to interfere with a target gene’s mRNA, or work with a different species, abm still has you covered with our Custom Services. Just tell our dedicated and highly experienced team what you want to accomplish, and we’ll do the gene synthesis, cloning, and adenoviral packaging for you!
Advantages of Adenoviruses:
✓ High transduction efficiency in most mammalian cells
✓ Infection is not dependent on active host cell division
✓ Low immunogenicity for high post-infection cell viability
✓ Broad host range
✓ High titers can be obtained
✓ A popular choice in vivo (and in vitro) applications
"We are very happy with the product and the turn around time."
Dr. Alexandra Duverger, University of Alabama at Birmingham
Service Details
Service Name
Size
Titer
CAT. NO.
PRICE
cDNA Adenovirus Production
1 ml
106 pfu/ml
C001
$650.00
siRNA/RNAi Adenovirus Production
1 ml
106 pfu/ml
C002
$650.00
Add-On Services
Service Name
Size
Titer
CAT. NO.
PRICE
Recombinant Adenovirus Amplification
2 x 1 ml
109 pfu/ml
C070
$450.00
Recombinant Adenovirus Amplification
2 x 1 ml
1010 pfu/ml
C071
$550.00
Recombinant Adenovirus Amplification
5 x 200 ul
1011 pfu/ml
C072
$850.00
Recombinant Adenovirus Amplification
5 x 200 ul
1012 pfu/ml
C005
$1550.00
Plaque Purification using ABMC1 cells
1 service
C004
$800.00
Plaque Assay for Adenovirus titer
1 service
C006
$450.00
RCA Assay using A549 cells
1 service
C007
$450.00
TCID50 Assay using 293 cells
1 service
C008
$350.00
293 Cell Line - low passages for viral production
Single Clone
C009
$250.00
Neutral Red
5 x 1.0 ml
0.33% [w/v]
G035
$35.00
Any additional assays required (such as those listed above) can be provided on request, at an additional cost. The viral titer will be calculated by end-point dilution assay by default. abm cannot guarantee resolution of titer discrepancies involving any other quantification methods.
For customer supplied vectors or seed stock, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
abm’s adenoviruses are replication-incompetent (-E1/-E3) and based on human adenovirus type 5 (Ad5).
Adenoviral cloning and packaging will be challenging for genes over 8.0 kb, but we will give it a try and you don't have to pay if the project fails. However, you do need to subclone into our pShuttle vector first; alternatively, we will do it for an extra charge.
The viral titer will be calculated by end-point dilution assay. Any additional assays required (such as those listed above) can be provided on request, at an additional cost.
We can accept a minimum of 125-250μl if your adenovirus is 10^6 pfu/ml to allow enough for further amplification. If you are able to supply more, we can offer a quicker turnaround time for your project. If your adenovirus stock is supplied at ≥10^8pfu/ml, please submit 250μl or more. Please ship the adenovirus stock on dry ice.
Our adenoviral vectors are currently only available as packaged viral particles. We do offer custom cloning services if further modifications are desirable.
McAllister, JM et al. "Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype." Proc Natl Acad Sci U S A 111(15):1519-27 (2014). DOI: 10.1073/pnas.1400574111PubMed: 24706793 Application: Transduction
Zhang, L et al. "Pivotal Role of MUC1 Glycosylation by Cigarette Smoke in Modulating Disruption of Airway Adherens Junctions In Vitro.." J Pathol : (2014). DOI: 10.1002/path.4375PubMed: 24838315 Application: Knock down
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