Anti-MBP Tag Antibody (Maltose Binding Protein)
Cat. No.
G079
Unit
100 μg
Price
$165.00
Specifications
Cat. No. G079
Name Anti-MBP Tag Antibody (Maltose Binding Protein)
Clone Polyclonal
Price $165.00
Unit 100 μg
Category Tag Antibodies
Application WB,IHC,IP
Raised In Rabbit
Clonality Polyclonal
Reactivity Other
Immunogen MBP epitope tag recombinant protein.
Isotype IgG
Concentration 100ug/100ul
Recommended Dilutions Optimal working dilutions should be determined experimentally by investigators. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 Immunoprecipitation: 5 µg/mg lysate Histo/Cytochemistry: 1:200
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Formulation Liquid
Specificity Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Guarantee abm guarantees that all our Anti-MBP Tag Antibody (Maltose Binding Protein) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Depositor Rockland Immunochemicals Inc.
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