OneScript® Plus Reverse Transcriptase
Cat. No.
G237
Unit
100 rxn
Price
$125.00
Specifications
SKU G237
Name OneScript® Plus Reverse Transcriptase
Unit 100 rxn
Description

(Equivalent to Cat. No. G177, G231, G232, G281, G282, G283, and G284.)

OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. 

OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features:

  • High thermostability at 50-55°C/122-131°F
  • Synthesize 15 kb RNA Templates
  • 10-15 minutes reaction time
  • Earn 2X the rewards points
  • OneScript® Plus cDNA Synthesis Kit (Cat. No. G236) also available
  • Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows

 

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ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Product Component Quantity
OneScript® Plus Reverse Transcriptase 100 rxn (100 µl)
5X RT Buffer 400 µl
 
2X the points for PCR Enzymes, Buffers, Solutions, and more!

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Note: This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript® Two-Step RT-PCR Kits and OneScript® Plus Two-Step RT-PCR Kits). Contact our customer service team technical@abmgood.com for more information.

 

 

Applications
  • Synthesizing cDNA from a ssRNA
  • DNA primer extension
  • Sequencing dsDNA
  • Constructing cDNA library
  • Constructing libraries for serial analysis for gene expression (SAGE)
  • Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
  • Producing template for use in RT-PCR or real-time RT-PCR
  • Labelling 3'-end of duplex DNA via end-filling reactions
  • Generating probes for hybridization
Storage Condition

Store at -20°C.

Documents
Supporting Protocol
MSDS
FAQs
There are no FAQs for this product yet!
References
  • Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
  • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
  • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
  • Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).
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