pLenti-5-UTR-GFP

Vector Name	pLenti-5-UTR-GFP
VectorType	Lentiviral Vector
Antibiotic Information	Puromycin
Sequencing Primers	5'-CTATAAAACCCAGCGGCGCGAC-3'
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg ggggagctca     
     2461 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg     
     2521 gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat cccgagccgg     
     2581 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg     
     2641 cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag gtaccgggga     
     2701 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct     
     2761 agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc cgcatcccga     
     2821 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct     
     2881 gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg aaaacaattc     
     2941 ggggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc     
     3001 ccgctagggg gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat     
     3061 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt     
     3121 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag     
     3181 gtaccgggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc     
     3241 ctcccccgct agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc     
     3301 cgcatcccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc     
     3361 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg     
     3421 aaaagaattc atagcagaca tacaacggac ggtgggccca gacccaggct gtgtagaccc     
     3481 agcccccccg ccccgcagtg cctaggtcac ccactaacgc cccaggcctt gtcttggctg     
     3541 ggcgtgactg ttaccctcaa aagcaggcag ctccagggta aaaggtgccc tgccctgtag     
     3601 agcccacctt ccttcccagg gctgcggctg ggtaggtttg tagccttcat cacgggccac     
     3661 ctccagccac tggaccgctg gcccctgccc tgtcctgggg agtgtggtcc tgcgacttct     
     3721 aagtggccgc aagccacctg actcccccaa caccacactc tacctctcaa gcccaggtct     
     3781 ctccctagtg acccacccag cacatttagc tagctgagcc ccacagccag aggtcctcag     
     3841 gccctgcttt cagggcagtt gctctgaagt cggcaagggg gagtgactgc ctggccactc     
     3901 catgccctcc aagagctcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg     
     3961 cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg     
     4021 tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg     
     4081 ggccgaaccg ggcactgccc agcgtggggc gcgggggcca cggcgcgcgc ccccagcccc     
     4141 cgggcccagc accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct     
     4201 cgctctcgct cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac     
     4261 tgtgcggcga agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag     
     4321 ttgcctttta tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg     
     4381 ccaccaccgc cgagaccgcg tccgccccga gatctcagag cctcgccttt catatcggcg     
     4441 cgccactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag     
     4501 actgcagctc gaggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc     
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg     
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct     
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg     
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt     
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa     
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga     
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat     
     4981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga     
     5041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt     
     5101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga     
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat     
     5221 ggacgagctg tacaaggggc accaccacca ccaccactga gtcgagtacc catacgacgt     
     5281 cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag     
     5341 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag     
     5401 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc     
     5461 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc     
     5521 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat     
     5581 tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc cacggtgcgc     
     5641 ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac     
     5701 taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt caccgagctg     
     5761 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac     
     5821 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc     
     5881 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg     
     5941 gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc     
     6001 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag     
     6061 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc     
     6121 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc     
     6181 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca acctctggat     
     6241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt     
     6301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc     
     6361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg     
     6421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc     
     6481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa     
     6541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat     
     6601 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc     
     6661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt     
     6721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga     
     6781 cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg ctaattcact     
     6841 cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct     
     6901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc     
     6961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc     
     7021 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta     
     7081 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg     
     7141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     
     7201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc     
     7261 atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt atgggtctgc     
     7321 attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta aggagaaaat     
     7381 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     
     8641 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg     
     8701 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa     
     8761 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg     
     8821 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg     
     8881 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc     
     8941 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg     
     9001 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg     
     9061 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa     
     9121 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct     
     9181 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga     
     9241 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc     
     9301 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac     
     9361 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc     
     9421 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact     
     9481 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga     
     9541 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg     
     9601 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga     
     9661 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga     
     9721 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt     
     9781 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat     
     9841 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat     
     9901 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac     
     9961 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc     
    10021 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga     
    10081 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     
    10141 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg     
    10201 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt

                            LOCUS       dna                     10250 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2439..2444
                     /dbxref="REBASE:ClaI"
     misc_binding    3425..3430
                     /dbxref="REBASE:EcoRI"
     ORF             3850..4563
                     /sequence="ORF_6 rf(6)"
     ORF             4430..5359
                     /sequence="ORF_5 rf(5)"
     misc_binding    4469..4474
                     /dbxref="REBASE:EcoRV"
     misc_binding    4490..4495
                     /dbxref="REBASE:BamHI"
     misc_binding    4496..4501
                     /dbxref="REBASE:XbaI"
     Reporter        4520..5236
                     /gene="EGFP reporter"
     ORF             4520..5260
                     /sequence="ORF_3 rf(2)"
     Promoter        5311..5579
                     /gene="SV40 prom"
     Rep_Origin      5478..5555
                     /gene="SV40 origin"
     ORF             5545..6213
                     /sequence="ORF_2 rf(1)"
     misc_binding    5558..5570
                     /dbxref="REBASE:SfiI"
     Marker          5614..6213
                     /gene="puro marker"
     Other Gene      6823..6875
                     /gene="delta_U3 other"
     Other Gene      6876..7056
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7562..8181
                     /gene="pBR322 origin"
     Promoter        8834..8883
                     /gene="NEOKAN prom"
     misc_binding    8941..8946
                     /dbxref="REBASE:BclI"
     ORF             8972..9766
                     /sequence="ORF_4 rf(2)"
     Marker          8975..9763
                     /gene="NTP_II marker"
     Rep_Origin      9871..10176
                     /gene="f1 origin"
BASE COUNT    2408 a   2828 c   2885 g   2129 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg ggggagctca     
     2461 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg     
     2521 gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat cccgagccgg     
     2581 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg     
     2641 cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag gtaccgggga     
     2701 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct     
     2761 agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc cgcatcccga     
     2821 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct     
     2881 gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg aaaacaattc     
     2941 ggggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc     
     3001 ccgctagggg gcagcagcga ccgcccgggg ctccgctccg gtccggcgct ccccccgcat     
     3061 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt     
     3121 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaag     
     3181 gtaccgggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc     
     3241 ctcccccgct agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc     
     3301 cgcatcccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc     
     3361 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg     
     3421 aaaagaattc atagcagaca tacaacggac ggtgggccca gacccaggct gtgtagaccc     
     3481 agcccccccg ccccgcagtg cctaggtcac ccactaacgc cccaggcctt gtcttggctg     
     3541 ggcgtgactg ttaccctcaa aagcaggcag ctccagggta aaaggtgccc tgccctgtag     
     3601 agcccacctt ccttcccagg gctgcggctg ggtaggtttg tagccttcat cacgggccac     
     3661 ctccagccac tggaccgctg gcccctgccc tgtcctgggg agtgtggtcc tgcgacttct     
     3721 aagtggccgc aagccacctg actcccccaa caccacactc tacctctcaa gcccaggtct     
     3781 ctccctagtg acccacccag cacatttagc tagctgagcc ccacagccag aggtcctcag     
     3841 gccctgcttt cagggcagtt gctctgaagt cggcaagggg gagtgactgc ctggccactc     
     3901 catgccctcc aagagctcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg     
     3961 cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg     
     4021 tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg     
     4081 ggccgaaccg ggcactgccc agcgtggggc gcgggggcca cggcgcgcgc ccccagcccc     
     4141 cgggcccagc accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct     
     4201 cgctctcgct cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac     
     4261 tgtgcggcga agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag     
     4321 ttgcctttta tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg     
     4381 ccaccaccgc cgagaccgcg tccgccccga gatctcagag cctcgccttt catatcggcg     
     4441 cgccactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag     
     4501 actgcagctc gaggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc     
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg     
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct     
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg     
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt     
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa     
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga     
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat     
     4981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga     
     5041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt     
     5101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga     
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat     
     5221 ggacgagctg tacaaggggc accaccacca ccaccactga gtcgagtacc catacgacgt     
     5281 cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag     
     5341 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag     
     5401 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc     
     5461 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc     
     5521 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat     
     5581 tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc cacggtgcgc     
     5641 ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac     
     5701 taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt caccgagctg     
     5761 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac     
     5821 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc     
     5881 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg     
     5941 gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc     
     6001 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag     
     6061 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc     
     6121 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc     
     6181 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca acctctggat     
     6241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt     
     6301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc     
     6361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg     
     6421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc     
     6481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa     
     6541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat     
     6601 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc     
     6661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt     
     6721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga     
     6781 cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg ctaattcact     
     6841 cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct     
     6901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc     
     6961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc     
     7021 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta     
     7081 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg     
     7141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     
     7201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc     
     7261 atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt atgggtctgc     
     7321 attaatgaat cggccaacga tcccggtgtg aaataccgca cagatgcgta aggagaaaat     
     7381 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     
     8641 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg     
     8701 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa     
     8761 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg     
     8821 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg     
     8881 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc     
     8941 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg     
     9001 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg     
     9061 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa     
     9121 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct     
     9181 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga     
     9241 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc     
     9301 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac     
     9361 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc     
     9421 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact     
     9481 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga     
     9541 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg     
     9601 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga     
     9661 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga     
     9721 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt     
     9781 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat     
     9841 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat     
     9901 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac     
     9961 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc     
    10021 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga     
    10081 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     
    10141 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg     
    10201 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt                
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1     ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
      AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61    gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
      CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

                                               Encap other(162,310)>>> 
                                               |
121   cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
      GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181   aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
      TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241   aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
      TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301   tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
      ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

          amp prom(365,393)<<< 
          |
361   ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
      CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

                                             HpaI 
                                             |
421   gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
      CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481   gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
      CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC

541   caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
      GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC

601   tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
      ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA

                                                            HIV-1_5_LTR other(715,895)>>> 
                                                            |
661   gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
      CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA

721   ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
      GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA

781   aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
      TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG

841   tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
      AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG

901   gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
      CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG

                                                   HIV-1_psi_pack other(1006,1050)>>> 
                                                   |
961   ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
      CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT

1021  ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
      AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC

                    NruI 
                    |
1081  ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
      CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT

1141  aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
      TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG

1201  tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
      ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT

1261  caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
      GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG

1321  aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
      TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT

1381  aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
      TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC

1441  ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
      CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT

                                                              RRE reg(1557,1790)>>> 
                                                              |
1501  gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
      CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT

                                             ORF_1 rf(1)(1600,2301)>>> 
                                             |
1561  gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
      CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC

1621  ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
      GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC

1681  agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
      TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG

1741  caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
      GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC

1801  ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
      CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA

1861  aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
      TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG

1921  aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
      TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA

1981  gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
      CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT

2041  aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
      TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT

2101  atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
      TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC

2161  tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
      AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT

2221  ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
      CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT

2281  aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
      TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC

2341  aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
      TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT

                                              ClaI 
                                              |
2401  aattcaaaattttcgggtttttcgaacctagccccgggatcgatcaattgggggagctca 2460
      TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCGGGGCCCTAGCTAGTTAACCCCCTCGAGT

2461  cggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggg 2520
      GCCCCTGTCGGGGGGGGGTTTCGGGGGTCCCTACATTAATGCAGGGAGGGGGCGATCCCC

2521  gcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccgg 2580
      CGTCGTCGCTGGCGGGCCCCGAGGCGAGGCCAGGCCGCGAGGGGGGCGTAGGGCTCGGCC

2581  cagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacg 2640
      GTCGCACGCCCCTGTCGGGCCCGTGCCCCTTCCACCGTGCCCTAGCGAAAGGAGACTTGC

2641  cttctcgctgctctttgagcctgcagacacctgggggatacggggaaaaggtaccgggga 2700
      GAAGAGCGACGAGAAACTCGGACGTCTGTGGACCCCCTATGCCCCTTTTCCATGGCCCCT

2701  gctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgct 2760
      CGAGTGCCCCTGTCGGGGGGGGGTTTCGGGGGTCCCTACATTAATGCAGGGAGGGGGCGA

2761  agggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccga 2820
      TCCCCCGTCGTCGCTGGCGGGCCCCGAGGCGAGGCCAGGCCGCGAGGGGGGCGTAGGGCT

2821  gccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctct 2880
      CGGCCGTCGCACGCCCCTGTCGGGCCCGTGCCCCTTCCACCGTGCCCTAGCGAAAGGAGA

2881  gaacgcttctcgctgctctttgagcctgcagacacctgggggatacggggaaaacaattc 2940
      CTTGCGAAGAGCGACGAGAAACTCGGACGTCTGTGGACCCCCTATGCCCCTTTTGTTAAG

2941  ggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctccc 3000
      CCCCTCGAGTGCCCCTGTCGGGGGGGGGTTTCGGGGGTCCCTACATTAATGCAGGGAGGG

3001  ccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcat 3060
      GGCGATCCCCCGTCGTCGCTGGCGGGCCCCGAGGCGAGGCCAGGCCGCGAGGGGGGCGTA

3061  cccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgcttt 3120
      GGGCTCGGCCGTCGCACGCCCCTGTCGGGCCCGTGCCCCTTCCACCGTGCCCTAGCGAAA

3121  cctctgaacgcttctcgctgctctttgagcctgcagacacctgggggatacggggaaaag 3180
      GGAGACTTGCGAAGAGCGACGAGAAACTCGGACGTCTGTGGACCCCCTATGCCCCTTTTC

3181  gtaccggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtcc 3240
      CATGGCCCCTCGAGTGCCCCTGTCGGGGGGGGGTTTCGGGGGTCCCTACATTAATGCAGG

3241  ctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccc 3300
      GAGGGGGCGATCCCCCGTCGTCGCTGGCGGGCCCCGAGGCGAGGCCAGGCCGCGAGGGGG

3301  cgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatc 3360
      GCGTAGGGCTCGGCCGTCGCACGCCCCTGTCGGGCCCGTGCCCCTTCCACCGTGCCCTAG

3361  gctttcctctgaacgcttctcgctgctctttgagcctgcagacacctgggggatacgggg 3420
      CGAAAGGAGACTTGCGAAGAGCGACGAGAAACTCGGACGTCTGTGGACCCCCTATGCCCC

           EcoRI 
           |
3421  aaaagaattcATAGCAGACATACAACGGACGGTGGGCCCAGACCCAGGCTGTGTAGACCC 3480
      TTTTCTTAAGTATCGTCTGTATGTTGCCTGCCACCCGGGTCTGGGTCCGACACATCTGGG

3481  AGCCCCCCCGCCCCGCAGTGCCTAGGTCACCCACTAACGCCCCAGGCCTTGTCTTGGCTG 3540
      TCGGGGGGGCGGGGCGTCACGGATCCAGTGGGTGATTGCGGGGTCCGGAACAGAACCGAC

3541  GGCGTGACTGTTACCCTCAAAAGCAGGCAGCTCCAGGGTAAAAGGTGCCCTGCCCTGTAG 3600
      CCGCACTGACAATGGGAGTTTTCGTCCGTCGAGGTCCCATTTTCCACGGGACGGGACATC

3601  AGCCCACCTTCCTTCCCAGGGCTGCGGCTGGGTAGGTTTGTAGCCTTCATCACGGGCCAC 3660
      TCGGGTGGAAGGAAGGGTCCCGACGCCGACCCATCCAAACATCGGAAGTAGTGCCCGGTG

3661  CTCCAGCCACTGGACCGCTGGCCCCTGCCCTGTCCTGGGGAGTGTGGTCCTGCGACTTCT 3720
      GAGGTCGGTGACCTGGCGACCGGGGACGGGACAGGACCCCTCACACCAGGACGCTGAAGA

3721  AAGTGGCCGCAAGCCACCTGACTCCCCCAACACCACACTCTACCTCTCAAGCCCAGGTCT 3780
      TTCACCGGCGTTCGGTGGACTGAGGGGGTTGTGGTGTGAGATGGAGAGTTCGGGTCCAGA

3781  CTCCCTAGTGACCCACCCAGCACATTTAGCTAGCTGAGCCCCACAGCCAGAGGTCCTCAG 3840
      GAGGGATCACTGGGTGGGTCGTGTAAATCGATCGACTCGGGGTGTCGGTCTCCAGGAGTC

               ORF_6 rf(6)(3850,4563)<<< 
               |
3841  GCCCTGCTTTCAGGGCAGTTGCTCTGAAGTCGGCAAGGGGGAGTGACTGCCTGGCCACTC 3900
      CGGGACGAAAGTCCCGTCAACGAGACTTCAGCCGTTCCCCCTCACTGACGGACCGGTGAG

3901  CATGCCCTCCAAGAGCTCCTTCTGCAGGAGCGTACAGAACCCAGGGCCCTGGCACCCGTG 3960
      GTACGGGAGGTTCTCGAGGAAGACGTCCTCGCATGTCTTGGGTCCCGGGACCGTGGGCAC

3961  CAGACCCTGGCCCACCCCACCTGGGCGCTCAGTGCCCAAGAGATGTCCACACCTAGGATG 4020
      GTCTGGGACCGGGTGGGGTGGACCCGCGAGTCACGGGTTCTCTACAGGTGTGGATCCTAC

4021  TCCCGCGGTGGGTGGGGGGCCCGAGAGACGGGCAGGCCGGGGGCAGGCCTGGCCATGCGG 4080
      AGGGCGCCACCCACCCCCCGGGCTCTCTGCCCGTCCGGCCCCCGTCCGGACCGGTACGCC

4081  GGCCGAACCGGGCACTGCCCAGCGTggggcgcgggggccacggcgcgcgcccccagcccc 4140
      CCGGCTTGGCCCGTGACGGGTCGCACCCCGCGCCCCCGGTGCCGCGCGCGGGGGTCGGGG

4141  cgggcccAGCACCCCAAGGCGGCCAACGCCAAAACTCTCCCTCCTCCTCTTCCTCAATCT 4200
      GCCCGGGTCGTGGGGTTCCGCCGGTTGCGGTTTTGAGAGGGAGGAGGAGAAGGAGTTAGA

4201  CGCTCTCGCTCTTTTTTTTTTTCGCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCAC 4260
      GCGAGAGCGAGAAAAAAAAAAAGCGTTTTCCTCCCCTCTCCCCCATTTTTTTACGACGTG

4261  TGTGCGGCGAAGCCGGTGAGTGAGCGGCGCGGGGCCAATCAGCGTGCGCCGTTCCGAAAG 4320
      ACACGCCGCTTCGGCCACTCACTCGCCGCGCCCCGGTTAGTCGCACGCGGCAAGGCTTTC

4321  TTGCCTTTTATGGCTCGAGCGGCCGCGGCGGCGCCCTATAAAACCCAGCGGCGCGACGCG 4380
      AACGGAAAATACCGAGCTCGCCGGCGCCGCCGCGGGATATTTTGGGTCGCCGCGCTGCGC

                                                       ORF_5 rf(5)(4430,5359)<<< 
                                                       |
4381  CCACCACCGCCGAGACCGCGTCCGCCCCGAGATCTCAGAGCCTCGCCTTTCATATcGGCG 4440
      GGTGGTGGCGGCTCTGGCGCAGGCGGGGCTCTAGAGTCTCGGAGCGGAAAGTATAGCCGC

                                     EcoRV              BamHI XbaI 
                                     |                  |     |
4441  CGCCactagtaccggtaggcctgtcgacgatatcgggcccgcggccgctggatcctctag 4500
      GCGGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATC

                         ORF_3 rf(2)(4520,5260)>>> 
                         |
                         EGFP reporter(4520,5236)>>> 
                         |
4501  actgcagctcgagGCCACCatggtgagcaagggcgaggagctgttcaccggggtggtgcc 4560
      TGACGTCGAGCTCCGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGG

4561  catcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgaggg 4620
      GTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCC

4621  cgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct 4680
      GCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGA

4681  gcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccg 4740
      CGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGC

4741  ctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgt 4800
      GATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCA

4801  ccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaa 4860
      GGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTT

4861  gttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagga 4920
      CAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCT

4921  cggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcat 4980
      GCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTA

4981  ggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgagga 5040
      CCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCT

5041  cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt 5100
      GCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCA

5101  gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacga 5160
      CGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCT

5161  gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcat 5220
      CTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTA

5221  ggacgagctgtacaaggggcaccaccaccaccaccactgagtcgagtacccatacgacgt 5280
      CCTGCTCGACATGTTCCCCGTGGTGGTGGTGGTGGTGACTCAGCTCATGGGTATGCTGCA

                                    SV40 prom(5311,5579)>>> 
                                    |
5281  cccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcag 5340
      GGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTC

5341  gcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccag 5400
      CGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTC

5401  gctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcc 5460
      CGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGG

                       SV40 origin(5478,5555)>>> 
                       |
5461  cgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccc 5520
      GCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGG

                                                   SfiI 
                                                   |
                              ORF_2 rf(1)(5545,6213)>>> 
                              |                    |
5521  atggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctat 5580
      TACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATA

                                       puro marker(5614,6213)>>> 
                                       |
5581  tccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgc 5640
      AGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCG

5641  ctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgac 5700
      GAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTG

5701  taccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctg 5760
      ATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGAC

5761  caagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgac 5820
      GTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTG

5821  ggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgcc 5880
      CCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGG

5881  gagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatg 5940
      CTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTAC

5941  gaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggc 6000
      CTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCG

6001  gtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggag 6060
      CAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTC

6061  gcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccc 6120
      CGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGG

6121  ttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgc 6180
      AAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCG

6181  acctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggat 6240
      TGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTA

6241  tacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgt 6300
      ATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACA

6301  ggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttc 6360
      CCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAG

6361  tcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcagg 6420
      AGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCC

6421  caacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcc 6480
      GTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGG

6481  accacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaa 6540
      TGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTT

6541  ctcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaat 6600
      GAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTA

6601  tccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacc 6660
      AGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGG

6661  tggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggacctt 6720
      ACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAA

6721  ccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcaga 6780
      GGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCT

                                                delta_U3 other(6823,6875)>>> 
                                                |
6781  cgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcact 6840
      GCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGA

                                         HIV-1_5_LTR other(6876,7056)>>> 
                                         |
6841  cccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatct 6900
      GGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGA

6901  gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgc 6960
      CTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACG

6961  cttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccc 7020
      GAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGG

7021  tcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttatta 7080
      AGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAAT

7081  ttcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattg 7140
      AAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAAC

7141  cagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttt 7200
      GTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAA

7201  tttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggc 7260
      AAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCG

7261  atctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgc 7320
      TAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACG

7321  attaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaat 7380
      TAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTA

7381  accgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggc 7440
      TGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCG

7441  tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 7500
      ACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCC

7501  ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg 7560
      TATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCC

       pBR322 origin(7562,8181)<<< 
       |
7561  ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac 7620
      GGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTG

7621  gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg 7680
      CGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGAC

7681  gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct 7740
      CTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGA

7741  ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 7800
      AAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCC

7801  tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct 7860
      ACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGA

7861  gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac 7920
      CGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTG

7921  tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt 7980
      ACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCA

7981  tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc 8040
      AGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAG

8041  tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 8100
      ACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGT

8101  ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat 8160
      GGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTA

8161  ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac 8220
      GAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTG

8221  gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt 8280
      CAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAA

8281  aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc 8340
      TTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGG

8341  aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg 8400
      TTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAAC

8401  cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg 8460
      GGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCAC

8461  ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc 8520
      GACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCG

8521  cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta 8580
      GTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGAT

8581  ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg 8640
      AATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAAC

8641  ttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtg 8700
      AACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCAC

8701  ctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaa 8760
      GACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTT

8761  gagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatg 8820
      CTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATAC

                   NEOKAN prom(8834,8883)>>> 
                   |
8821  gacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctg 8880
      CTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGAC

8881  caaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctc 8940
      GTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAG

                                        NTP_II marker(8975,9763)>>> 
                                        |
       BclI                          ORF_4 rf(2)(8972,9766)>>> 
       |                             |  |
8941  tgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcagg 9000
      ACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCC

9001  ttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcgg 9060
      AAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCC

9061  ctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaa 9120
      GACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTT

9121  gaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggct 9180
      CTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGA

9181  ggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggga 9240
      CCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCT

9241  ctggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgc 9300
      GACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACG

9301  cgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctac 9360
      GCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATG

9361  ctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagc 9420
      GACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCG

9421  cggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaact 9480
      GCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGA

9481  gttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcga 9540
      CAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCT

9541  tgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtgg 9600
      ACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACC

9601  ccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctga 9660
      GGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACT

9661  agagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccga 9720
      TCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCT

9721  ttcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatt 9780
      AAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAA

9781  tttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaat 9840
      AAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTA

                                    f1 origin(9871,10176)<<< 
                                    |
9841  caaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactat 9900
      GTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATA

9901  taaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccac 9960
      ATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTG

9961  tacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatc 10020
      ATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAG

10021 ggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcga 10080
      CCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCT

10081 gaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtca 10140
      CTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGT

10141 cgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcg 10200
      GCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGC

10201 ccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10250
      GGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA

                            LOCUS       dna                     10250 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2439..2444
                     /dbxref="REBASE:ClaI"
     misc_binding    3425..3430
                     /dbxref="REBASE:EcoRI"
     ORF             3850..4563
                     /sequence="ORF_6 rf(6)"
     ORF             4430..5359
                     /sequence="ORF_5 rf(5)"
     misc_binding    4469..4474
                     /dbxref="REBASE:EcoRV"
     misc_binding    4490..4495
                     /dbxref="REBASE:BamHI"
     misc_binding    4496..4501
                     /dbxref="REBASE:XbaI"
     Reporter        4520..5236
                     /gene="EGFP reporter"
     ORF             4520..5260
                     /sequence="ORF_3 rf(2)"
     Promoter        5311..5579
                     /gene="SV40 prom"
     Rep_Origin      5478..5555
                     /gene="SV40 origin"
     ORF             5545..6213
                     /sequence="ORF_2 rf(1)"
     misc_binding    5558..5570
                     /dbxref="REBASE:SfiI"
     Marker          5614..6213
                     /gene="puro marker"
     Other Gene      6823..6875
                     /gene="delta_U3 other"
     Other Gene      6876..7056
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7562..8181
                     /gene="pBR322 origin"
     Promoter        8834..8883
                     /gene="NEOKAN prom"
     misc_binding    8941..8946
                     /dbxref="REBASE:BclI"
     ORF             8972..9766
                     /sequence="ORF_4 rf(2)"
     Marker          8975..9763
                     /gene="NTP_II marker"
     Rep_Origin      9871..10176
                     /gene="f1 origin"
BASE COUNT    2408 a   2828 c   2885 g   2129 t    0 others