pLenti-5-UTR-Luc
| Vector Name | pLenti-5-UTR-Luc |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | 5'-CTATAAAACCCAGCGGCGCGAC-3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg agtacttacg
2461 taggtacccc agtgtggtgg cctgcaggtg aattcatagc agacatacaa cggacggtgg
2521 gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag gtcacccact
2581 aacgccccag gccttgtctt ggctgggcgt gactgttacc ctcaaaagca ggcagctcca
2641 gggtaaaagg tgccctgccc tgtagagccc accttccttc ccagggctgc ggctgggtag
2701 gtttgtagcc ttcatcacgg gccacctcca gccactggac cgctggcccc tgccctgtcc
2761 tggggagtgt ggtcctgcga cttctaagtg gccgcaagcc acctgactcc cccaacacca
2821 cactctacct ctcaagccca ggtctctccc tagtgaccca cccagcacat ttagctagct
2881 gagccccaca gccagaggtc ctcaggccct gctttcaggg cagttgctct gaagtcggca
2941 agggggagtg actgcctggc cactccatgc cctccaagag ctccttctgc aggagcgtac
3001 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc
3061 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag
3121 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt ggggcgcggg
3181 ggccacggcg cgcgccccca gcccccgggc ccagcacccc aaggcggcca acgccaaaac
3241 tctccctcct cctcttcctc aatctcgctc tcgctctttt tttttttcgc aaaaggaggg
3301 gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg gtgagtgagc ggcgcggggc
3361 caatcagcgt gcgccgttcc gaaagttgcc ttttatggct cgagcggccg cggcggcgcc
3421 ctataaaacc cagcggcgcg acgcgccacc accgccgaga ccgcgtccgc cccgagatct
3481 cagagcctcg cctttcatat cggcgcgcca ctagtaccgg taggcctgtc gacgatatcg
3541 ggcccgcggc cgctggatcc gccaccatgg aagatgccaa aaacattaag aagggcccag
3601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
3661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
3721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
3781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
3841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
3901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
3961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
4021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
4081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
4141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
4201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
4261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
4321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
4381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
4441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
4501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
4561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
4621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
4681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
4741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
4801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
4861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
4921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
4981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
5041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
5101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
5161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaac
5221 tcgagcctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa
5281 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
5341 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
5401 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
5461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
5521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
5581 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
5641 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
5701 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
5761 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
5821 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
5881 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg
5941 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
6001 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
6061 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
6121 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
6181 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac
6241 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
6301 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
6361 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
6421 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
6481 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
6541 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
6601 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
6661 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
6721 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc
6781 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt
6841 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
6901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
6961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
7021 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
7081 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
7141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
7201 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag
7261 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc
7321 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
7381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
7441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
7501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
7561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
7621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
7681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
7741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
7801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
7861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
7921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
7981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
8041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
8101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
8161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
8221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
8281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
8341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
8401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
8461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
8521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
8581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga
8641 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
8701 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
8761 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
8821 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
8881 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
8941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
9001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
9061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
9121 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
9181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
9241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
9301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
9361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
9421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
9481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
9541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
9601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
9661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
9721 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
9781 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
9841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
9901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
9961 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
10021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
10081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
10141 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
10201 taattaacat catcaataat atacctt
LOCUS dna 10227 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2439..2444
/dbxref="REBASE:ClaI"
misc_binding 2463..2468
/dbxref="REBASE:KpnI"
misc_binding 2490..2495
/dbxref="REBASE:EcoRI"
ORF 2915..3586
/sequence="ORF_6 rf(6)"
misc_binding 3534..3539
/dbxref="REBASE:EcoRV"
misc_binding 3555..3560
/dbxref="REBASE:BamHI"
ORF 3567..5219
/sequence="ORF_3 rf(3)"
ORF 4563..5336
/sequence="ORF_5 rf(5)"
Promoter 5288..5556
/gene="SV40 prom"
Rep_Origin 5455..5532
/gene="SV40 origin"
ORF 5522..6190
/sequence="ORF_2 rf(2)"
misc_binding 5535..5547
/dbxref="REBASE:SfiI"
Marker 5591..6190
/gene="puro marker"
Other Gene 6800..6852
/gene="delta_U3 other"
Other Gene 6853..7033
/gene="HIV-1_5_LTR other"
Rep_Origin 7539..8158
/gene="pBR322 origin"
Promoter 8811..8860
/gene="NEOKAN prom"
ORF 8949..9743
/sequence="ORF_4 rf(3)"
Marker 8952..9740
/gene="NTP_II marker"
Rep_Origin 9848..10153
/gene="f1 origin"
BASE COUNT 2472 a 2727 c 2824 g 2204 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gccccgggat cgatcaattg agtacttacg
2461 taggtacccc agtgtggtgg cctgcaggtg aattcatagc agacatacaa cggacggtgg
2521 gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag gtcacccact
2581 aacgccccag gccttgtctt ggctgggcgt gactgttacc ctcaaaagca ggcagctcca
2641 gggtaaaagg tgccctgccc tgtagagccc accttccttc ccagggctgc ggctgggtag
2701 gtttgtagcc ttcatcacgg gccacctcca gccactggac cgctggcccc tgccctgtcc
2761 tggggagtgt ggtcctgcga cttctaagtg gccgcaagcc acctgactcc cccaacacca
2821 cactctacct ctcaagccca ggtctctccc tagtgaccca cccagcacat ttagctagct
2881 gagccccaca gccagaggtc ctcaggccct gctttcaggg cagttgctct gaagtcggca
2941 agggggagtg actgcctggc cactccatgc cctccaagag ctccttctgc aggagcgtac
3001 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc
3061 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag
3121 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt ggggcgcggg
3181 ggccacggcg cgcgccccca gcccccgggc ccagcacccc aaggcggcca acgccaaaac
3241 tctccctcct cctcttcctc aatctcgctc tcgctctttt tttttttcgc aaaaggaggg
3301 gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg gtgagtgagc ggcgcggggc
3361 caatcagcgt gcgccgttcc gaaagttgcc ttttatggct cgagcggccg cggcggcgcc
3421 ctataaaacc cagcggcgcg acgcgccacc accgccgaga ccgcgtccgc cccgagatct
3481 cagagcctcg cctttcatat cggcgcgcca ctagtaccgg taggcctgtc gacgatatcg
3541 ggcccgcggc cgctggatcc gccaccatgg aagatgccaa aaacattaag aagggcccag
3601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
3661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
3721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
3781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
3841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
3901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
3961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
4021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
4081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
4141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
4201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
4261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
4321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
4381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
4441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
4501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
4561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
4621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
4681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
4741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
4801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
4861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
4921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
4981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
5041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
5101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
5161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaac
5221 tcgagcctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa
5281 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
5341 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
5401 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
5461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
5521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
5581 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
5641 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
5701 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
5761 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
5821 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
5881 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg
5941 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
6001 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
6061 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
6121 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
6181 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac
6241 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
6301 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
6361 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
6421 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
6481 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
6541 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
6601 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
6661 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
6721 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc
6781 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt
6841 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
6901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
6961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
7021 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
7081 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
7141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
7201 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag
7261 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc
7321 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
7381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
7441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
7501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
7561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
7621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
7681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
7741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
7801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
7861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
7921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
7981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
8041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
8101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
8161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
8221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
8281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
8341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
8401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
8461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
8521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
8581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga
8641 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
8701 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
8761 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
8821 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
8881 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
8941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
9001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
9061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
9121 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
9181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
9241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
9301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
9361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
9421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
9481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
9541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
9601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
9661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
9721 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
9781 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
9841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
9901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
9961 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
10021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
10081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
10141 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
10201 taattaacat catcaataat atacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 TTTTGGATTGAAGCCAATATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGT 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 GGGAACGGGGCGGGTGACGTAGTAGTGTGGCGGAAGTGTGATGTTGCAAGTGTGGCGGAA 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 CACATGTAAGCGACGGATGTGGCAAAAGTGACGTTTTTGGTGTGCGCCGGTGTACACAGG 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 AAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTAGTAAATTTGGGCGTAACCGAGT 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 AAGATTTGGCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTGAAATCTGAATAATTTTG 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 TGTTACTCATAGCGCGTAATACGGCAGACCTCAGCGCTAGATTATTGAAGCATTTATCAG 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
421 GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTAACTATAACGGTCCTAAGGTA 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 GCGAAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAG 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 CAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGG 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 TACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACT 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 GAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCT 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 CTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTT 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 AAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGAC 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 TCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGC 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 GCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTC 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 GGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAA 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 TTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGG 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 GGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATA 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 AATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCC 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 TGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGA 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 CAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATC 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 AAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACA 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 AAAGTAAGACCACCGCACAGCAAGCCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAG 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 GGACATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTA 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGA 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 GCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACG 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTG 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 AGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTC 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 CAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGG 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 GGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 AAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAAC 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 AATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAAT 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 GAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACA 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 AATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 ATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCG 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 TTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAA 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 GGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGA 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 AAGCTTGGGATTCGAATTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAG 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 AATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACAAAAACAAATTACAAA 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
ClaI
|
2401 AATTCAAAATTTTCGGGTTTTTCGAACCTAGCCCCGGGATCGATCAATTGAGTACTTACG 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCGGGGCCCTAGCTAGTTAACTCATGAATGC
KpnI EcoRI
| |
2461 TAGGTACCCCAGTGTGGTGGCCTGCAGGTGAATTCATAGCAGACATACAACGGACGGTGG 2520
ATCCATGGGGTCACACCACCGGACGTCCACTTAAGTATCGTCTGTATGTTGCCTGCCACC
2521 GCCCAGACCCAGGCTGTGTAGACCCAGCCCCCCCGCCCCGCAGTGCCTAGGTCACCCACT 2580
CGGGTCTGGGTCCGACACATCTGGGTCGGGGGGGCGGGGCGTCACGGATCCAGTGGGTGA
2581 AACGCCCCAGGCCTTGTCTTGGCTGGGCGTGACTGTTACCCTCAAAAGCAGGCAGCTCCA 2640
TTGCGGGGTCCGGAACAGAACCGACCCGCACTGACAATGGGAGTTTTCGTCCGTCGAGGT
2641 GGGTAAAAGGTGCCCTGCCCTGTAGAGCCCACCTTCCTTCCCAGGGCTGCGGCTGGGTAG 2700
CCCATTTTCCACGGGACGGGACATCTCGGGTGGAAGGAAGGGTCCCGACGCCGACCCATC
2701 GTTTGTAGCCTTCATCACGGGCCACCTCCAGCCACTGGACCGCTGGCCCCTGCCCTGTCC 2760
CAAACATCGGAAGTAGTGCCCGGTGGAGGTCGGTGACCTGGCGACCGGGGACGGGACAGG
2761 TGGGGAGTGTGGTCCTGCGACTTCTAAGTGGCCGCAAGCCACCTGACTCCCCCAACACCA 2820
ACCCCTCACACCAGGACGCTGAAGATTCACCGGCGTTCGGTGGACTGAGGGGGTTGTGGT
2821 CACTCTACCTCTCAAGCCCAGGTCTCTCCCTAGTGACCCACCCAGCACATTTAGCTAGCT 2880
GTGAGATGGAGAGTTCGGGTCCAGAGAGGGATCACTGGGTGGGTCGTGTAAATCGATCGA
ORF_6 rf(6)(2915,3586)<<<
|
2881 GAGCCCCACAGCCAGAGGTCCTCAGGCCCTGCTTTCAGGGCAGTTGCTCTGAAGTCGGCA 2940
CTCGGGGTGTCGGTCTCCAGGAGTCCGGGACGAAAGTCCCGTCAACGAGACTTCAGCCGT
2941 AGGGGGAGTGACTGCCTGGCCACTCCATGCCCTCCAAGAGCTCCTTCTGCAGGAGCGTAC 3000
TCCCCCTCACTGACGGACCGGTGAGGTACGGGAGGTTCTCGAGGAAGACGTCCTCGCATG
3001 AGAACCCAGGGCCCTGGCACCCGTGCAGACCCTGGCCCACCCCACCTGGGCGCTCAGTGC 3060
TCTTGGGTCCCGGGACCGTGGGCACGTCTGGGACCGGGTGGGGTGGACCCGCGAGTCACG
3061 CCAAGAGATGTCCACACCTAGGATGTCCCGCGGTGGGTGGGGGGCCCGAGAGACGGGCAG 3120
GGTTCTCTACAGGTGTGGATCCTACAGGGCGCCACCCACCCCCCGGGCTCTCTGCCCGTC
3121 GCCGGGGGCAGGCCTGGCCATGCGGGGCCGAACCGGGCACTGCCCAGCGTggggcgcggg 3180
CGGCCCCCGTCCGGACCGGTACGCCCCGGCTTGGCCCGTGACGGGTCGCACCCCGCGCCC
3181 ggccacggcgcgcgcccccagcccccgggcccAGCACCCCAAGGCGGCCAACGCCAAAAC 3240
CCGGTGCCGCGCGCGGGGGTCGGGGGCCCGGGTCGTGGGGTTCCGCCGGTTGCGGTTTTG
3241 TCTCCCTCCTCCTCTTCCTCAATCTCGCTCTCGCTCTTTTTTTTTTTCGCAAAAGGAGGG 3300
AGAGGGAGGAGGAGAAGGAGTTAGAGCGAGAGCGAGAAAAAAAAAAAGCGTTTTCCTCCC
3301 GAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAAGCCGGTGAGTGAGCGGCGCGGGGC 3360
CTCTCCCCCATTTTTTTACGACGTGACACGCCGCTTCGGCCACTCACTCGCCGCGCCCCG
3361 CAATCAGCGTGCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGCGGCCGCGGCGGCGCC 3420
GTTAGTCGCACGCGGCAAGGCTTTCAACGGAAAATACCGAGCTCGCCGGCGCCGCCGCGG
3421 CTATAAAACCCAGCGGCGCGACGCGCCACCACCGCCGAGACCGCGTCCGCCCCGAGATCT 3480
GATATTTTGGGTCGCCGCGCTGCGCGGTGGTGGCGGCTCTGGCGCAGGCGGGGCTCTAGA
EcoRV
|
3481 CAGAGCCTCGCCTTTCATATcGGCGCGCCACTAGTACCGGTAGGCCTGTCGACGATATCG 3540
GTCTCGGAGCGGAAAGTATAGCCGCGCGGTGATCATGGCCATCCGGACAGCTGCTATAGC
BamHI ORF_3 rf(3)(3567,5219)>>>
| |
3541 GGCCCGCGGCCGCTGGATCCGCCACCATGGAAGATGCCAAAAACATTAAGAAGGGCCCAG 3600
CCGGGCGCCGGCGACCTAGGCGGTGGTACCTTCTACGGTTTTTGTAATTCTTCCCGGGTC
3601 CGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGC 3660
GCGGTAAGATGGGTGAGCTTCTGCCCTGGCGGCCGCTCGTCGACGTGTTTCGGTACTTCG
3661 GCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTA 3720
CGATGCGGGACCACGGGCCGTGGTAGCGGAAATGGCTGCGTGTATAGCTCCACCTGTAAT
3721 CCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGC 3780
GGATGCGGCTCATGAAGCTCTACTCGCAAGCCGACCGTCTTCGATACTTCGCGATACCCG
3781 TGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCG 3840
ACTTATGTTTGGTAGCCTAGCACCACACGTCGCTCTTATCGAACGTCAAGAAGTACGGGC
3841 TGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGC 3900
ACAACCCACGGGACAAGTAGCCACACCGACACCGGGGTCGATTGCTGTAGATGTTGCTCG
3901 GCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAG 3960
CGCTCGACGACTTGTCGTACCCGTAGTCGGTCGGGTGGCAGCATAAGCACTCGTTCTTTC
3961 GGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCA 4020
CCGACGTTTTCTAGGAGTTGCACGTTTTCTTCGATGGCTAGTATGTTTTCTAGTAGTAGT
4021 TGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATT 4080
ACCTATCGTTCTGGCTGATGGTCCCGAAGGTTTCGTACATGTGGAAGCACTGAAGGGTAA
4081 TGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCA 4140
ACGGTGGGCCGAAGTTGCTCATGCTGAAGCACGGGCTCTCGAAGCTGGCCCTGTTTTGGT
4141 TCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGC 4200
AGCGGGACTAGTACTTGTCATCACCGTCATGGCCTAACGGGTTCCCGCATCGGGATGGCG
4201 ACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCA 4260
TGGCGTGGCGAACACAGGCTAAGTCAGTACGGGCGCTGGGGTAGAAGCCGTTGGTCTAGT
4261 TCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCA 4320
AGGGGCTGTGGCGATAGGAGTCGCACCACGGTAAAGTGGTGCCGAAGCCGTACAAGTGGT
4321 CGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGC 4380
GCGACCCGATGAACTAGACGCCGAAAGCCCAGCACGAGTACATGGCGAAGCTCCTCCTCG
4381 TATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTAT 4440
ATAAGAACGCGTCGAACGTTCTGATATTCTAAGTTAGACGGGACGACCACGGGTGTGATA
4441 TTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGA 4500
AATCGAAGAAGCGATTCTCGTGAGAGTAGCTGTTCATGCTGGATTCGTTGAACGTGCTCT
4501 TCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCC 4560
AGCGGTCGCCGCCCCGCGGCGAGTCGTTCCTCCATCCACTCCGGCACCGGTTTGCGAAGG
ORF_5 rf(5)(4563,5336)<<<
|
4561 ACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCA 4620
TGGATGGTCCGTAGGCGGTCCCGATGCCGGACTGTCTTTGTTGGTCGCGGTAAGACTAGT
4621 CCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTA 4680
GGGGGCTTCCCCTGCTGTTCGGACCGCGTCATCCGTTCCACCACGGGAAGAAGCTCCGAT
4681 AGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCG 4740
TCCACCACCTGAACCTGTGGCCATTCTGTGACCCACACTTGGTCGCGCCGCTCGACACGC
4741 TCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCA 4800
AGGCACCGGGGTACTAGTACTCGCCGATGCAATTGTTGGGGCTCCGATGTTTGCGAGAGT
4801 TCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACT 4860
AGCTGTTCCTGCCGACCGACGTGTCGCCGCTGTAGCGGATGACCCTGCTCCTGCTCGTGA
4861 TCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAG 4920
AGAAGTAGCACCTGGCCGACTTCTCGGACTAGTTTATGTTCCCGATGGTCCATCGGGGTC
4921 CCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCC 4980
GGCTTGACCTCTCGTAGGACGACGTTGTGGGGTTGTAGAAGCTGCGGCCCCAGCGGCCGG
4981 TGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAA 5040
ACGGGCTGCTGCTACGGCCGCTCGACGGGCGGCGTCAGCAGCACGACCTTGTGCCATTTT
5041 CCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGC 5100
GGTACTGGCTCTTCCTCTAGCACCTGATACACCGGTCGGTCCAATGTTGGCGGTTCTTCG
5101 TGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACG 5160
ACGCGCCACCACAACACAAGCACCTGCTCCACGGATTTCCTGACTGGCCGTTCAACCTGC
5161 CCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGTAAC 5220
GGGCGTTCTAGGCGCTCTAAGAGTAATTCCGGTTCTTCCCGCCGTTCTAGCGGCACATTG
5221 TCGAGCCTAGACTGCAGCTCGAGTACCCATACGACGTCCCAGACTACGCTTGAGTTTAAA 5280
AGCTCGGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTT
SV40 prom(5288,5556)>>>
|
5281 CACGCGTGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCAT 5340
GTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTA
5341 CTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATG 5400
GAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATAC
SV40 origin(5455,5532)>>>
|
5401 CAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCG 5460
GTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGC
5461 CCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATT 5520
GGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAA
SfiI
|
ORF_2 rf(2)(5522,6190)>>>
| |
5521 TATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTT 5580
ATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAA
puro marker(5591,6190)>>>
|
5581 TTTGGAGGCCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCC 5640
AAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGG
5641 TCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGT 5700
AGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCA
5701 GGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGT 5760
CCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCA
5761 CGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGAC 5820
GCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTG
5821 CACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGA 5880
GTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCT
5881 GTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGGCTCCTGGCGCCGCACCG 5940
CAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGC
5941 GCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAA 6000
CGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTT
6001 GGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCC 6060
CCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGG
6061 CGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCAC 6120
GCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTG
6121 CGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCC 6180
GCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGG
6181 CGGTGCCTGAACGCGTTCCGGAAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGAC 6240
GCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTG
6241 TGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTT 6300
ACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAA
6301 GTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTT 6360
CATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAA
6361 GCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGT 6420
CGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACA
6421 GTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGG 6480
CAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCC
6481 GACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCG 6540
CTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGC
6541 CTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCT 6600
GACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGA
6601 GACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTT 6660
CTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAA
6661 CTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGC 6720
GACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCG
6721 TCTGCGGCCTCTTCCGCGTCTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCC 6780
AGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGG
delta_U3 other(6800,6852)>>>
|
6781 GCCTCCCCGCCTGTCCGGATGGAAGGGCTAATTCACTCCCAACGAATACAAGATCTGCTT 6840
CGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAA
HIV-1_5_LTR other(6853,7033)>>>
|
6841 TTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAA 6900
AAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATT
6901 CTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGT 6960
GATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACA
6961 GCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGG 7020
CGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACC
7021 AAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAG 7080
TTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTC
7081 AAATGAATATCAGAGAGTGAGAGGAACTTGTTTATTGCAGCTTATAATGGTTACAAATAA 7140
TTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATT
7141 AGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGT 7200
TCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCA
7201 TTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCATCTATGTCGGGTGCGGAGAAAG 7260
AACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTC
7261 AGGTAATGAAATGGCATTATGGGTATTATGGGTCTGCATTAATGAATCGGCCAACGATCC 7320
TCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGG
7321 CGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCT 7380
GCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGA
7381 TCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCAC 7440
AGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTG
7441 TCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGA 7500
AGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACT
pBR322 origin(7539,8158)<<<
|
7501 GCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCAT 7560
CGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTA
7561 AGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAAC 7620
TCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTG
7621 CCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCT 7680
GGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGA
7681 GTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCG 7740
CAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGC
7741 CTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 7800
GAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGAC
7801 GGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 7860
CCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCA
7861 CTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGG 7920
GAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCC
7921 ATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTAC 7980
TAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATG
7981 GGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA 8040
CCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCT
8041 AAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTT 8100
TTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAA
8101 GTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTT 8160
CAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAA
8161 TCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGA 8220
AGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCT
8221 TTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATC 8280
AATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAG
8281 TAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCT 8340
ATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGA
8341 ATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATA 8400
TAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTAT
8401 ACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCA 8460
TGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGT
8461 CGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA 8520
GCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCT
8521 AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGA 8580
TCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCT
8581 GTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGAAAAAGGATCTTCACCTAGA 8640
CATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCT
8641 TCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCT 8700
AGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGA
8701 ACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTG 8760
TGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCAC
NEOKAN prom(8811,8860)>>>
|
8761 GGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGC 8820
CCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACG
8821 CAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCT 8880
GTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGA
8881 CGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGAT 8940
GCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTA
NTP_II marker(8952,9740)>>>
|
ORF_4 rf(3)(8949,9743)>>>
| |
8941 CGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGA 9000
GCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCT
9001 GGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCC 9060
CCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGG
9061 GGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGA 9120
CCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACT
9121 ATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCG 9180
TACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGC
9181 CAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGC 9240
GTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACG
9241 CGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTG 9300
GCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGAC
9301 ATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGA 9360
TACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCT
9361 AACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATC 9420
TTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAG
9421 TGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCA 9480
ACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGT
9481 TGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGG 9540
ACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACC
9541 TGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCT 9600
ACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGA
9601 ATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTG 9660
TAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGAC
9661 ACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATC 9720
TGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAG
9721 GCCTTCTTGACGAGTTCTTCTGAATTTTGTTAAAATTTTTGTTAAATCAGCTCATTTTTT 9780
CGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAA
9781 AACCAATAGGCCGAAATCGGCAACATCCCTTATAAATCAAAAGAATAGACCGCGATAGGG 9840
TTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCC
f1 origin(9848,10153)<<<
|
9841 TTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTC 9900
AACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAG
9901 AAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCA 9960
TTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGT
9961 AGTTTTTTGCGGTCGAGGTGCCGTAAAGCTCTAAATCGGAACCCTAAAGGGAGCCCCCGA 10020
TCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCT
10021 TTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAA 10080
AAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTT
10081 GGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCC 10140
CCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGG
10141 GCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGCCATTCAGGATCGAATTAATTCT 10200
CGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGA
10201 TAATTAACATCATCAATAATATACCTT 10227
ATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10227 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2439..2444
/dbxref="REBASE:ClaI"
misc_binding 2463..2468
/dbxref="REBASE:KpnI"
misc_binding 2490..2495
/dbxref="REBASE:EcoRI"
ORF 2915..3586
/sequence="ORF_6 rf(6)"
misc_binding 3534..3539
/dbxref="REBASE:EcoRV"
misc_binding 3555..3560
/dbxref="REBASE:BamHI"
ORF 3567..5219
/sequence="ORF_3 rf(3)"
ORF 4563..5336
/sequence="ORF_5 rf(5)"
Promoter 5288..5556
/gene="SV40 prom"
Rep_Origin 5455..5532
/gene="SV40 origin"
ORF 5522..6190
/sequence="ORF_2 rf(2)"
misc_binding 5535..5547
/dbxref="REBASE:SfiI"
Marker 5591..6190
/gene="puro marker"
Other Gene 6800..6852
/gene="delta_U3 other"
Other Gene 6853..7033
/gene="HIV-1_5_LTR other"
Rep_Origin 7539..8158
/gene="pBR322 origin"
Promoter 8811..8860
/gene="NEOKAN prom"
ORF 8949..9743
/sequence="ORF_4 rf(3)"
Marker 8952..9740
/gene="NTP_II marker"
Rep_Origin 9848..10153
/gene="f1 origin"
BASE COUNT 2472 a 2727 c 2824 g 2204 t 0 others