Recombinant Human CXCL14 (BRAK) (E. coli)
Cat. No.
Z102375
Unit
Price
$205.00
Specifications
| Description | Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-α and MIP-β sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells. |
| SKU | Z102375 |
| Unit quantity | 20 µg |
| Family | Chemokine-alpha Family (CXC) |
| Aliases | Chemokine BRAK, MIP-2G, Small-inducible cytokine B14 |
| Gene Symbol | CXCL14 |
| Gene ID | 9547 |
| Accession Number | O95715 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 9.6 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2µm filtered solution containing 20mM Tris pH 8.5 and 1M NaCl |
| Function | The ED50 is 0.25-1.25 ng/mL as determined by its ability to induce calcium flux in activated monocytes |
| Reconstitution | A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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