pLenti-EF1a-GFP-2A-Puro

Vector Name	pLenti-EF1a-GFP-2A-Puro
VectorType	Plasmid
Antibiotic Information	Puromycin
Sequencing Primers	EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc     
     1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg     
     1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     
     1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     
     2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg     
     2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg     
     2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa     
     2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     
     2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     
     2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     
     2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     
     2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     
     2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     
     2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     
     2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg     
     2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac     
     2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg     
     2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     
     2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     
     2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     
     3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag     
     3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata     
     3121 tcgggcccgc ggccgctgga tcctctagag gtgtggaaag tccccaggct ccccagcagg     
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg     
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc     
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca     
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt     
     3421 ccagaagtag tgaggaggct tttttggagg cctgtcgagg ccaccatgga gagcgacgag     
     3481 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag     
     3541 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg     
     3601 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac     
     3661 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc     
     3721 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac     
     3781 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc     
     3841 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc     
     3901 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc     
     3961 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc     
     4021 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag     
     4081 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc     
     4141 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga     
     4201 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag     
     4261 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac     
     4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac     
     4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg     
     4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc     
     4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg     
     4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg     
     4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag     
     4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg     
     4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc     
     4801 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc     
     4861 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga     
     4921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     
     4981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     
     5041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     
     5101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     
     5161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     
     5221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     
     5281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     
     5341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     
     5401 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt     
     5461 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc     
     5521 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg     
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag     
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag     
     5701 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg     
     5761 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca     
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga     
     5941 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac     
     6001 ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     
     6061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     
     6121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     
     6181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     
     6241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     
     6301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     
     6361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     
     6421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     
     6481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     
     6541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     
     6601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     
     6661 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     
     6721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     
     6781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     
     6841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggcc     
     6901 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag     
     6961 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca     
     7021 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg     
     7081 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg     
     7141 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga     
     7201 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     
     7261 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     
     7321 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     
     7381 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     
     7441 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     
     7501 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     
     7561 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     
     7621 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     
     7681 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     
     7741 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     
     7801 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     
     7861 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     
     7921 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     
     7981 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg     
     8041 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     
     8101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     
     8161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     
     8221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     
     8281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     
     8341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     
     8401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     
     8461 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atataccttt     
     8521 tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg     
     8581 ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac     
     8641 acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt gtacacagga     
     8701 agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg taaccgagta     
     8761 agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga ataattttgt     
     8821 gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta tttagaaaaa     
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg taactataac     
     8941 ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg caacatggta     
     9001 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg     
     9061 gaagtaaggt ggtacgat

                            LOCUS       dna                      9078 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     ORF             1775..2755
                     /sequence="ORF_4 rf(6)"
     Promoter        1838..3015
                     /gene="EF1a prom"
     misc_binding    2802..2807
                     /dbxref="REBASE:XhoI"
     misc_binding    3087..3092
                     /dbxref="REBASE:EcoRI"
     misc_binding    3111..3116
                     /dbxref="REBASE:HincII"
     misc_binding    3117..3122
                     /dbxref="REBASE:EcoRV"
     misc_binding    3129..3136
                     /dbxref="REBASE:NotI"
     misc_binding    3138..3143
                     /dbxref="REBASE:BamHI"
     misc_binding    3144..3149
                     /dbxref="REBASE:XbaI"
     Promoter        3150..3418
                     /gene="SV40 prom"
     Rep_Origin      3317..3394
                     /gene="SV40 origin"
     misc_binding    3397..3409
                     /dbxref="REBASE:SfiI"
     ORF             3466..4875
                     /sequence="ORF_2 rf(1)"
     Marker          4276..4875
                     /gene="puro marker"
     Other Gene      5485..5537
                     /gene="delta_U3 other"
     Other Gene      5538..5718
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6225..6844
                     /gene="pBR322 origin"
     Promoter        7102..7151
                     /gene="NEOKAN prom"
     misc_binding    7209..7214
                     /dbxref="REBASE:BclI"
     ORF             7240..8034
                     /sequence="ORF_3 rf(1)"
     Marker          7243..8031
                     /gene="NTP_II marker"
     Rep_Origin      8139..8445
                     /gene="f1 origin"
     Other Gene      8500..8601
                     /gene="RITR other"
     Other Gene      8500..8601
                     /gene="LITR other"
     Other Gene      8681..8829
                     /gene="Encap other"
BASE COUNT    2129 a   2324 c   2603 g   2022 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc     
     1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg     
     1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     
     1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     
     2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg     
     2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg     
     2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa     
     2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     
     2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     
     2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     
     2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     
     2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     
     2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     
     2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     
     2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg     
     2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac     
     2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg     
     2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     
     2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     
     2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     
     3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag     
     3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata     
     3121 tcgggcccgc ggccgctgga tcctctagag gtgtggaaag tccccaggct ccccagcagg     
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg     
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc     
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca     
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt     
     3421 ccagaagtag tgaggaggct tttttggagg cctgtcgagg ccaccatgga gagcgacgag     
     3481 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag     
     3541 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg     
     3601 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac     
     3661 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc     
     3721 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac     
     3781 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc     
     3841 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc     
     3901 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc     
     3961 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc     
     4021 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag     
     4081 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc     
     4141 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga     
     4201 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag     
     4261 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac     
     4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac     
     4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg     
     4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc     
     4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg     
     4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg     
     4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag     
     4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg     
     4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc     
     4801 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc     
     4861 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga     
     4921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     
     4981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     
     5041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     
     5101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     
     5161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     
     5221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     
     5281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     
     5341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     
     5401 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt     
     5461 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc     
     5521 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg     
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag     
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag     
     5701 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg     
     5761 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca     
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga     
     5941 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac     
     6001 ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     
     6061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     
     6121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     
     6181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     
     6241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     
     6301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     
     6361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     
     6421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     
     6481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     
     6541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     
     6601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     
     6661 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     
     6721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     
     6781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     
     6841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggcc     
     6901 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag     
     6961 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca     
     7021 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg     
     7081 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg     
     7141 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga     
     7201 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     
     7261 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     
     7321 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     
     7381 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     
     7441 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     
     7501 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     
     7561 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     
     7621 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     
     7681 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     
     7741 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     
     7801 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     
     7861 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     
     7921 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     
     7981 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg     
     8041 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     
     8101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     
     8161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     
     8221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     
     8281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     
     8341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     
     8401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     
     8461 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atataccttt     
     8521 tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg     
     8581 ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac     
     8641 acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt gtacacagga     
     8701 agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg taaccgagta     
     8761 agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga ataattttgt     
     8821 gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta tttagaaaaa     
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg taactataac     
     8941 ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg caacatggta     
     9001 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg     
     9061 gaagtaaggt ggtacgat

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1     cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
      GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC

                                                      HIV-1_5_LTR other(109,289)>>> 
                                                      |
61    ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
      GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC

121   ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
      AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA

181   caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
      GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA

241   aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
      TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT

301   acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
      TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC

                                             HIV-1_psi_pack other(400,444)>>> 
                                             |
361   ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
      GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT

421   ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
      GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT

481   ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
      AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT

541   aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
      TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC

601   aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
      TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA

661   cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
      GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT

721   tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
      ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT

781   agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
      TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA

841   tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
      ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG

                                                        RRE reg(951,1184)>>> 
                                                        |
901   accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
      TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC

                                       ORF_1 rf(1)(994,1695)>>> 
                                       |
961   ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
      AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC

1021  gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
      CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA

1081  attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
      TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT

1141  agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
      TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG

1201  tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
      AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA

1261  ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
      GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG

1321  acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
      TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT

1381  gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
      CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC

1441  ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
      GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA

1501  tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
      AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC

1561  acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
      TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT

1621  gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
      CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA

1681  gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
      CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA

                                        ORF_4 rf(6)(1775,2755)<<< 
                                        |
1741  agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
      TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT

                                           EF1a prom(1838,3015)>>> 
                                           |
1801  aaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtcagtgggc 1860
      TTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCG

1861  agagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccgg 1920
      TCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCC

1921  tgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcct 1980
      ACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGA

1981  ttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttt 2040
      AAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAA

2041  tcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctgg 2100
      AGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACC

2101  cctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtg 2160
      GGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCAC

2161  attcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa 2220
      TAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATT

2221  ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtg 2280
      CCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCAC

2281  cgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaa 2340
      GCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATT

2341  aatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggc 2400
      TTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCG

2401  caagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcg 2460
      GTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGC

2461  tcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgg 2520
      AGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCC

2521  gggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgcc 2580
      CCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGG

2581  ccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg 2640
      GGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGC

2641  cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcg 2700
      GAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGC

2701  ggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgac 2760
      CCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTG

                                                XhoI 
                                                |
2761  tccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg 2820
      AGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGC

2821  tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtg 2880
      AGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCAC

2881  gagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcccttttt 2940
      CTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAA

2941  gagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttcca 3000
      CTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGT

3001  tttcaggtgtcgtgaggaatttcgactgctagctggtacctgaattgagtacttacgtag 3060
      AAAGTCCACAGCACTCCTTAAAGCTGACGATCGACCATGGACTTAACTCATGAATGCATC

                                 EcoRI                     HincII 
                                 |                         |
3061  gtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctgtcgacgata 3120
      CATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTAT

     EcoRV      NotI    BamHI XbaI SV40 prom(3150,3418)>>> 
     |          |       |     |    |
3121  tcgggcccgcggccgctggatcctctagaggtgtggaaagtccccaggctccccagcagg 3180
      AGCCCGGGCGCCGGCGACCTAGGAGATCTCCACACCTTTCAGGGGTCCGAGGGGTCGTCC

3181  cagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccagg 3240
      GTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCC

3241  ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtccc 3300
      GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGG

                      SV40 origin(3317,3394)>>> 
                      |
3301  gcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccca 3360
      CGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGT

                                                  SfiI 
                                                  |
3361  tggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctatt 3420
      ACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAA

                                                   ORF_2 rf(1)(3466,4875)>>> 
                                                   |
3421  ccagaagtagtgaggaggcttttttggaggcctgtcgaggccaccatggagagcgacgag 3480
      GGTCTTCATCACTCCTCCGAAAAAACCTCCGGACAGCTCCGGTGGTACCTCTCGCTGCTC

3481  agcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggag 3540
      TCGCCGGACGGGCGGTACCTCTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCGCACCTC

3541  ttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatg 3600
      AAGCTCGACCACCCGCCGCCTCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTGTTCTAC

3601  aagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctac 3660
      TTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGGATGGACGACTCGGTGCACTACCCGATG

3661  ggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatc 3720
      CCGAAGATGGTGAAGCCGTGGATGGGGTCGCCGATGCTCTTGGGGAAGGACGTGCGGTAG

3721  aacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcac 3780
      TTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCACGACGTG

3781  gtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggc 3840
      CACTCGAAGTCGATGGCGATGCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCACCACCCG

3841  accggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccacc 3900
      TGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTGCGGTGG

3901  gtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttc 3960
      CACCTCGTGGACGTGGGGTACCCGCTATTGCACGACCACCCGTCGAAGCGGGCGTGGAAG

3961  agcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagc 4020
      TCGGACGCGCTGCCGCCGATGATGTCGAAGCACCACCTGTCGGTGTACGTGAAGTTCTCG

4021  gccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggag 4080
      CGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCGCACCTC

4081  gagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagaccccc 4140
      CTCGACGTGTCGTTGTGGCTCGACCCGTAGCACCTCATGGTCGTGCGGAAGTTCTGGGGG

4141  atcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccgga 4200
      TAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGGCGGCCT

4201  cccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggag 4260
      GGGCCGAGGTGGCCTAGAGCGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTC

                     puro marker(4276,4875)>>> 
                     |
4261  gagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgac 4320
      CTCTTAGGGCCGGGATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTG

4321  gtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccac 4380
      CAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTG

4381  accgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacg 4440
      TGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGC

4441  cgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtc 4500
      GCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAG

4501  tggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatg 4560
      ACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTAC

4561  gccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccg 4620
      CGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGC

4621  caccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccag 4680
      GTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTC

4681  ggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggg 4740
      CCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCC

4741  gtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggc 4800
      CACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCG

4801  ttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgc 4860
      AAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCG

4861  aagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaaga 4920
      TTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCT

4921  ttgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatg 4980
      AACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTAC

4981  cctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcc 5040
      GGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGG

5041  tggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgc 5100
      ACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACG

5101  actgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctt 5160
      TGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAA

5161  tccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcctt 5220
      AGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAA

5221  gcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgggg 5280
      CGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCC

5281  aagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacg 5340
      TTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGC

5341  tccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctg 5400
      AGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGAC

5401  ccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcccttt 5460
      GGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAA

                              delta_U3 other(5485,5537)>>> 
                              |
5461  gggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatc 5520
      CCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAG

                       HIV-1_5_LTR other(5538,5718)>>> 
                       |
5521  tgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctg 5580
      ACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGAC

5581  gctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtag 5640
      CGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATC

5641  tgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcag 5700
      ACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTC

5701  tgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttg 5760
      ACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAAC

5761  caaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttaca 5820
      GTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGT

5821  aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagtt 5880
      TTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAA

5881  gtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcgga 5940
      CACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCT

5941  gaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaac 6000
      CTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTG

6001  ggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctct 6060
      CCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGA

6061  tccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatca 6120
      AGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGT

6121  gctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac 6180
      CGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTG

                                                  pBR322 origin(6225,6844)<<< 
                                                  |
6181  atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttt 6240
      TACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAA

6241  ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtgg 6300
      AAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACC

6301  cgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgc 6360
      GCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCG

6361  tctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagc 6420
      AGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCG

6421  gtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcc 6480
      CACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGG

6481  aagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac 6540
      TTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTG

6541  tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggt 6600
      ATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCA

6601  aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcct 6660
      TTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGA

6661  aactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacc 6720
      TTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGG

6721  ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt 6780
      AAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCA

6781  ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttg 6840
      AAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAAC

6841  atcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggcc 6900
      TAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGG

6901  atgagattatcaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcag 6960
      TACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTC

6961  aaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgca 7020
      TTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGT

7021  agcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcg 7080
      TCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGC

                           NEOKAN prom(7102,7151)>>> 
                           |
7081  gttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttggg 7140
      CAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCC

7141  aagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcagggga 7200
      TTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCT

                                                NTP_II marker(7243,8031)>>> 
                                                |
               BclI                          ORF_3 rf(1)(7240,8034)>>> 
               |                             |  |
7201  tcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattg 7260
      AGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAAC

7261  cacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacag 7320
      GTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTC

7321  acaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctt 7380
      TGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAA

7381  tttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggcta 7440
      AAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGAT

7441  tcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcg 7500
      AGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGC

7501  ggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacctt 7560
      CCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAA

7561  gctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgat 7620
      CGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTA

7621  ccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcgg 7680
      GGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCC

7681  atggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcca 7740
      TACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGT

7741  gccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacc 7800
      CGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGG

7801  catggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatc 7860
      GTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAG

7861  gactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgat 7920
      CTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTA

7921  attgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgcc 7980
      TAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGG

7981  gctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttg 8040
      CGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAAC

8041  ttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatccc 8100
      AATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGG

                                            f1 origin(8139,8445)<<< 
                                            |
8101  ttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagag 8160
      AATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTC

8161  tccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcga 8220
      AGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCT

8221  tggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagc 8280
      ACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCG

8281  actaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaa 8340
      TGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTT

8341  cgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgt 8400
      GCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACA

8401  agcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgc 8460
      TCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCG

                                             RITR other(8500,8601)<<< 
                                             |
                                             LITR other(8500,8601)>>> 
                                             |
8461  gtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatataccttt 8520
      CAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAA

8521  tttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtg 8580
      AAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCAC

8581  ggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaac 8640
      CCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTG

                                              Encap other(8681,8829)>>> 
                                              |
8641  acatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagga 8700
      TGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCT

8701  agtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagta 8760
      TCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCAT

8761  agatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgt 8820
      TCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACA

8821  gttactcatagcgcgtaatactctcatgagcggatacatatttgaatgtatttagaaaaa 8880
      CAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTT

8881  taaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtaactataac 8940
      ATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTG

8941  ggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggta 9000
      CCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCAT

9001  acgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtg 9060
      TGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCAC

9061  gaagtaaggtggtacgat 9078
      CTTCATTCCACCATGCTA

                            LOCUS       dna                      9078 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     ORF             1775..2755
                     /sequence="ORF_4 rf(6)"
     Promoter        1838..3015
                     /gene="EF1a prom"
     misc_binding    2802..2807
                     /dbxref="REBASE:XhoI"
     misc_binding    3087..3092
                     /dbxref="REBASE:EcoRI"
     misc_binding    3111..3116
                     /dbxref="REBASE:HincII"
     misc_binding    3117..3122
                     /dbxref="REBASE:EcoRV"
     misc_binding    3129..3136
                     /dbxref="REBASE:NotI"
     misc_binding    3138..3143
                     /dbxref="REBASE:BamHI"
     misc_binding    3144..3149
                     /dbxref="REBASE:XbaI"
     Promoter        3150..3418
                     /gene="SV40 prom"
     Rep_Origin      3317..3394
                     /gene="SV40 origin"
     misc_binding    3397..3409
                     /dbxref="REBASE:SfiI"
     ORF             3466..4875
                     /sequence="ORF_2 rf(1)"
     Marker          4276..4875
                     /gene="puro marker"
     Other Gene      5485..5537
                     /gene="delta_U3 other"
     Other Gene      5538..5718
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6225..6844
                     /gene="pBR322 origin"
     Promoter        7102..7151
                     /gene="NEOKAN prom"
     misc_binding    7209..7214
                     /dbxref="REBASE:BclI"
     ORF             7240..8034
                     /sequence="ORF_3 rf(1)"
     Marker          7243..8031
                     /gene="NTP_II marker"
     Rep_Origin      8139..8445
                     /gene="f1 origin"
     Other Gene      8500..8601
                     /gene="RITR other"
     Other Gene      8500..8601
                     /gene="LITR other"
     Other Gene      8681..8829
                     /gene="Encap other"
BASE COUNT    2129 a   2324 c   2603 g   2022 t    0 others