pLenti-GFP
| Vector Name | pLenti-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Neomycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ORIGIN
1 TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG
51 CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG
101 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG
151 ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG
201 TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC
251 CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG
301 TGTTACTCAT AGCGCGTAAT ACGGCAGACC TCAGCGCTAG ATTATTGAAG
351 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
401 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
451 CCTGACGTTA ACTATAACGG TCCTAAGGTA GCGAAAGCTC AGATCTGGAT
501 CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA
551 TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA
601 GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT
651 AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA
701 GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG
751 GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG
801 TCTGACATGG ATTGGACGAA CCACTGAATT GCCGCATTGC AGAGATATTG
851 TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG GTTAGACCAG
901 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC
951 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT
1001 GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA
1051 ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA
1101 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG
1151 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG
1201 GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA
1251 ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA
1301 ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG
1351 CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG
1401 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT
1451 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA
1501 AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT
1551 AAGACCACCG CACAGCAAGC GCCGCTGATC TTCAGACCTG GAGGAGGAGA
1601 TATGAGGGAC AATTGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT
1651 TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA
1701 GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA
1751 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC
1801 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG
1851 CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG
1901 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA
1951 GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG
2001 TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT
2051 CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT
2101 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC
2151 AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC
2201 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG
2251 CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG
2301 TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG
2351 AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA
2401 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGATAAG
2451 CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA
2501 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
2551 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC
2601 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
2651 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
2701 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
2751 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT
2801 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
2851 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC
2901 GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
2951 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG
3001 ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG CTAGCGTTTA
3051 AACTTAAGCT TGGTACCGCG GGCCCGGGAT CCACCGGTCG CCACCATGGT
3101 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
3151 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
3201 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG
3251 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG
3301 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
3351 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
3401 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
3451 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
3501 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA
3551 TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC
3601 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
3651 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
3701 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA
3751 TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
3801 GAGCTGTACA AGTAAAGCGG CCGCTCGAGC ATCATCACCA TCACCATTGA
3851 TGATCCGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
3901 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
3951 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
4001 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
4051 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT
4101 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT
4151 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGCGAC CAGATCTAAT
4201 GCGTGCTAGC ACGCATTAGA TCGGTCGCTA GCGACCAGAT CTAATGCGTG
4251 CTAGCGACCA GATCTAATGC GTGCTAGGCT TTTGCAAAAA GCTTGGGCTG
4301 CAGGTCGAGG CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
4351 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
4401 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG
4451 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
4501 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
4551 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA
4601 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
4651 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
4701 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC
4751 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
4801 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
4851 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG
4901 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
4951 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
5001 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG
5051 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG
5101 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
5151 CTGGGGTTCG ATATCTAGCG ACCAGATCTA ATTGCGTGCA AGGCAGCTGT
5201 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC
5251 ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG
5301 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT
5351 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
5401 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA
5451 GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA
5501 TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGGGCC
5551 TGACCAGATC TAATGCGTAG GCCGTTTAAA CCGCTGATCA GCCTCGACTG
5601 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTG CCCGGGCGCG ATCGCTGCCC
5651 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT
5701 CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
5751 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA
5801 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
5851 AAAGAACCAG CAGATCGATC TGCATCTATG TCGGGTGCGG AGAAAGAGGT
5901 AATGAAATGG CATTATGGGT ATTATGGGTC TGCATTAATG AATCGGCCAA
5951 CGATCCCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
6001 CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
6051 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
6101 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
6151 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
6201 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
6251 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
6301 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
6351 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
6401 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
6451 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
6501 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
6551 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
6601 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
6651 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
6701 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
6751 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
6801 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
6851 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
6901 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
6951 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
7001 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
7051 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT
7101 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
7151 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
7201 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
7251 TTGTTGAAAA AGGATCTTCA CCTAGATCCT TTTCACGTAG AAAGCCAGTC
7301 CGCAGAAACG GTGCTGACCC CGGATGAATG TCAGCTACTG GGCTATCTGG
7351 ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG CAGGTAGCTT GCAGTGGGCT
7401 TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA AGCGAACCGG
7451 AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA
7501 AACTGGATGG CTTTCTCGCC GCCAAGGATC TGATGGCGCA GGGGATCAAG
7551 CTCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG
7601 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
7651 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
7701 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
7751 CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
7801 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
7851 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC
7901 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
7951 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
8001 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
8051 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
8101 AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG
8151 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT
8201 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG
8251 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
8301 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT
8351 TCTATCGCCT TCTTGACGAG TTCTTCTGAA TTTTGTTAAA ATTTTTGTTA
8401 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAC ATCCCTTATA
8451 AATCAAAAGA ATAGACCGCG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
8501 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC
8551 CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC AAATCAAGTT
8601 TTTTGCGGTC GAGGTGCCGT AAAGCTCTAA ATCGGAACCC TAAAGGGAGC
8651 CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
8701 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG
8751 TCACGCTGCG CGTAACCACC ACACCCGCGC GCTTAATGCG CCGCTACAGG
8801 GCGCGTCCAT TCGCCATTCA GGATCGAATT AATTCTTAAT TAACATCATC
8851 AATAATATAC CTT
//
LOCUS dna 8799 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_1 rf(2)"
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
ORF 2927..3850
/sequence="ORF_2 rf(2)"
misc_binding 3062..3067
/dbxref="REBASE:KpnI"
misc_binding 3070..3075
/dbxref="REBASE:ApaI"
misc_binding 3077..3082
/dbxref="REBASE:BamHI"
Reporter 3096..3812
/gene="EGFP reporter"
ORF 3096..3815
/sequence="ORF_3 rf(3)"
misc_binding 3817..3824
/dbxref="REBASE:NotI"
misc_binding 3824..3829
/dbxref="REBASE:XhoI"
Tag 3830..3847
/gene="6xHis tag"
Regulatory_Seq 3863..4099
/gene="SV40ER reg"
Promoter 3886..4154
/gene="SV40 prom"
Rep_Origin 4053..4130
/gene="SV40 origin"
misc_binding 4133..4145
/dbxref="REBASE:SfiI"
misc_binding 4184..4189
/dbxref="REBASE:StuI"
ORF 4284..5078
/sequence="ORF_4 rf(3)"
Marker 4287..5075
/gene="NTP_II marker"
misc_binding 5096..5101
/dbxref="REBASE:EcoRV"
Other Gene 5172..5224
/gene="delta_U3 other"
Other Gene 5225..5405
/gene="HIV-1_5_LTR other"
Rep_Origin 6111..6730
/gene="pBR322 origin"
Promoter 7383..7432
/gene="NEOKAN prom"
ORF 7521..8315
/sequence="ORF_5 rf(3)"
Marker 7524..8312
/gene="NTP_II marker"
Rep_Origin 8420..8725
/gene="f1 origin"
BASE COUNT 2231 a 2151 c 2432 g 1985 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct
3061 tggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
3121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
3181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
3241 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
3301 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
3361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
3421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
3481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
3541 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
3601 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
3661 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
3721 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
3781 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgctcgagc atcatcacca
3841 tcaccattga tgatgatccc cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
3901 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gtgaccagat
4201 ctaatgcgta ggcccttttg caaaaagctt gggctgcagg tcgaggcgga tctgatcaag
4261 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
4321 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
4381 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
4441 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
4501 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
4561 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
4621 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
4681 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
4741 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
4801 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
4861 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
4921 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
4981 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
5041 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgatat
5101 ctagcgacca gatctaattg cgtgcaaggc agctgtagat cttagccact ttttaaaaga
5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
5221 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
5281 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
5341 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
5401 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
5461 atcagagagt gagaggaact tgggcctgac cagatctaat gcgtaggccg tttaaaccgc
5521 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg ggcgcgatcg
5581 ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
5641 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
5701 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
5761 ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca tctatgtcgg
5821 gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca ttaatgaatc
5881 ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
5941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
6001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
6061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
6121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
6181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
6241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
6301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
6361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
6421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
6481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
6541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
6601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
6661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
6721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
6781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
6841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
6901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
6961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
7021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
7081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
7141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga
7201 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
7261 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
7321 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
7381 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
7441 ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
7501 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
7561 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
7621 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
7681 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
7741 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
7801 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
7861 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
7921 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
7981 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
8041 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
8101 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
8161 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
8221 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
8281 tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc
8341 agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc aaaagaatag
8401 accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
8461 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
8521 tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa
8581 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
8641 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
8701 accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
8761 cgaattaatt cttaattaac atcatcaata atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtct 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAATTACATCAGA
661 tatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaa 720
ATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTT
721 ggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgcctta 780
CCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGCACGGAAT
781 ttaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgc 840
AATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGGCGTAACG
HIV-1_5_LTR other(880,1060)>>>
|
841 agagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccag 900
TCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACCAATCTGGTC
901 atctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagc 960
TAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCG
961 ttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagaga 1020
AACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCT
1021 tccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggact 1080
AGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGA
1081 tgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgc 1140
ACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCG
HIV-1_psi_pack other(1171,1215)>>>
|
1141 gcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggag 1200
CGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTC
1201 gctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgc 1260
CGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCG
NruI
|
1261 gatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatag 1320
CTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATATC
1321 tatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcag 1380
ATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTC
1381 aaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaac 1440
TTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTG
1441 ttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataa 1500
AATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCTCTATT
1501 aagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccg 1560
TTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGC
1561 cacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaag 1620
GTGTCGTTCGCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACTCTTC
1621 tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggc 1680
ACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGTGGTTCCG
RRE reg(1723,1956)>>>
|
1681 aaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgg 1740
TTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGAACC
ORF_1 rf(2)(1766,2449)>>>
|
1741 gttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggc 1800
CAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCG
1801 cagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc 1860
GTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGATAACTCCG
1861 gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcct 1920
CGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTTCTTAGGA
1921 ggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaa 1980
CCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTT
1981 actcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaaca 2040
TGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAGACCTTGT
2041 gatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt 2100
CTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTGTTCGAA
2101 aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattatt 2160
TTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAA
2161 ggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggta 2220
CCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGACACCAT
2221 tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgt 2280
ATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAAACGACA
2281 actttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct 2340
TGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGA
2341 cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagaga 2400
GGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCTCTCTCT
2401 cagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagtt 2460
GTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTATTCGAACCCTCAA
2461 ccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgccc 2520
GGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGG
2521 attgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacg 2580
TAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGC
NdeI
|
2581 tcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat 2640
AGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATA
2641 gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgccca 2700
CGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGT
2701 gtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctat 2760
CATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATA
2761 taccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacg 2820
ATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGC
2821 gggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatca 2880
CCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGT
ORF_2 rf(2)(2927,3850)>>>
|
CMV prom(2911,2992)>>>
| |
2881 acgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcg 2940
TGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGC
2941 tgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggag 3000
ACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTC
3001 acgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagct 3060
TGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCGATCGCAAATTTGAATTCGA
BamHI ORF_3 rf(3)(3096,3815)>>>
| |
KpnI ApaI EGFP reporter(3096,3812)>>>
| | | |
3061 tggtaccGCGGGCCCGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGT 3120
ACCATGGCGCCCGGGCCCTAGGTGGCCAGCGGTGGTACCACTCGTTCCCGCTCCTCGACA
3121 TCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA 3180
AGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGT
3181 GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCT 3240
CGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGA
3241 GCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCG 3300
CGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGC
3301 TGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCA 3360
ACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGT
3361 TGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGA 3420
ACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCT
3421 CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCA 3480
GGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGT
3481 TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCC 3540
AGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGG
3541 ACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCC 3600
TGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGG
3601 GCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCA 3660
CGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGT
3661 TCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGA 3720
AGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACT
3721 GCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCG 3780
CGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGC
NotI XhoI 6xHis tag(3830,3847)>>>
| | |
3781 GGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGctcgagcatcatcacca 3840
CCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTTCGCCGGCGAGCTCGTAGTAGTGGT
SV40 prom(3886,4154)>>>
|
SV40ER reg(3863,4099)<<<
| |
3841 tcaccattgatgatgatccccggctgtggaatgtgtgtcagttagggtgtggaaagtccc 3900
AGTGGTAACTACTACTAGGGGCCGACACCTTACACACAGTCAATCCCACACCTTTCAGGG
3901 caggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggt 3960
GTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCA
3961 gtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagt 4020
CACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCA
SV40 origin(4053,4130)>>>
|
4021 cagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccg 4080
GTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGC
4081 cccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcct 4140
GGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGA
SfiI StuI
| |
4141 cggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagtgaccagat 4200
GCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCACTGGTCTA
4201 ctaatgcgtaggcccttttgcaaaaagcttgggctgcaggtcgaggcggatctgatcaag 4260
GATTACGCATCCGGGAAAACGTTTTTCGAACCCGACGTCCAGCTCCGCCTAGACTAGTTC
NTP_II marker(4287,5075)>>>
|
ORF_4 rf(3)(4284,5078)>>>
| |
4261 agacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccgg 4320
TCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCC
4321 ccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctg 4380
GGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGAC
4381 atgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacc 4440
TACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGG
4441 tgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacga 4500
ACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCT
4501 cgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgc 4560
GCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACG
4561 tattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaag 4620
ATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTC
4621 tatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccat 4680
ATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTA
4681 tcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttg 4740
AGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAAC
4741 tcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcca 4800
AGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGT
4801 ggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgct 4860
CCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGA
4861 tgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgg 4920
ACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACC
4921 gtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttg 4980
CACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAAC
4981 gcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagc 5040
CGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCG
EcoRV
|
5041 gcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgatat 5100
CGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCGCCCTGAGACCCCAAGCTATA
5101 ctagcgaccagatctaattgcgtgcaaggcagctgtagatcttagccactttttaaaaga 5160
GATCGCTGGTCTAGATTAACGCACGTTCCGTCGACATCTAGAATCGGTGAAAAATTTTCT
delta_U3 other(5172,5224)>>>
|
5161 aaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttg 5220
TTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTTCTGTTCTAGACGAAAAACGAAC
HIV-1_5_LTR other(5225,5405)>>>
|
5221 tactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaa 5280
ATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTT
5281 cccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtct 5340
GGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGA
5341 gttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctc 5400
CAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAG
5401 tagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaat 5460
ATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTA
5461 atcagagagtgagaggaacttgggcctgaccagatctaatgcgtaggccgtttaaaccgc 5520
TAGTCTCTCACTCTCCTTGAACCCGGACTGGTCTAGATTACGCATCCGGCAAATTTGGCG
5521 tgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccgggcgcgatcg 5580
ACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAACGGGCCCGCGCTAGC
5581 ctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttccta 5640
GACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGAT
5641 ataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtgg 5700
TATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACC
5701 ggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgc 5760
CCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACG
5761 ggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcatctatgtcgg 5820
CCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGTAGATACAGCC
5821 gtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatc 5880
CACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAG
5881 ggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcagg 5940
CCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCC
5941 cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcg 6000
GCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGC
6001 gtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagga 6060
CATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCT
pBR322 origin(6111,6730)<<<
|
6061 aagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctg 6120
TTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGAC
6121 gcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcag 6180
CGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTC
6181 aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctc 6240
TCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAG
6241 gtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcg 6300
CACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGC
6301 ggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgtt 6360
CCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAA
6361 cgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc 6420
GCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGG
6421 ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcc 6480
CCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGG
6481 actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtgg 6540
TGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACC
6541 tggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagcca 6600
ACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGT
6601 gttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc 6660
CAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCG
6661 ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagat 6720
CCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTA
6721 cctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggatt 6780
GGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAA
6781 ttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagt 6840
AACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCA
6841 tttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatc 6900
AAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAG
6901 agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcccc 6960
TCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGG
6961 gtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgata 7020
CAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTAT
7021 ccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagg 7080
GGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCC
7081 gccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc 7140
CGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACG
7141 cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaagga 7200
GCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCT
7201 tcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccgga 7260
AGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCT
7261 tgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcagg 7320
ACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCC
7321 tagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcg 7380
ATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGC
NEOKAN prom(7383,7432)>>>
|
7381 aaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaact 7440
TTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGA
7441 ggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagaga 7500
CCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCT
NTP_II marker(7524,8312)>>>
|
ORF_5 rf(3)(7521,8315)>>>
| |
7501 caggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccg 7560
GTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGC
7561 cttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatg 7620
GAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTAC
7621 ccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgt 7680
GGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACA
7681 ccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgg 7740
GGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCC
7741 gcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctat 7800
CGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATA
7801 tgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtat 7860
ACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATA
7861 ccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcg 7920
GGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGC
7921 accaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcg 7980
TGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGC
7981 atcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggc 8040
TAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCG
8041 tcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgc 8100
AGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACG
8101 cgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 8160
GCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCAC
8161 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcg 8220
ACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGC
8221 gcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgca 8280
CGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGT
8281 tcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatc 8340
AGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAG
8341 agctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatag 8400
TCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATC
f1 origin(8420,8725)<<<
|
8401 accgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtg 8460
TGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCAC
8461 gactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaacca 8520
CTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGT
8521 tcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaa 8580
AGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTT
8581 gggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggg 8640
CCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCC
8641 aagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgta 8700
TTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCAT
8701 accaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggat 8760
TGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTA
8761 cgaattaattcttaattaacatcatcaataatatacctt 8799
GCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 8799 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_1 rf(2)"
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
ORF 2927..3850
/sequence="ORF_2 rf(2)"
misc_binding 3062..3067
/dbxref="REBASE:KpnI"
misc_binding 3070..3075
/dbxref="REBASE:ApaI"
misc_binding 3077..3082
/dbxref="REBASE:BamHI"
Reporter 3096..3812
/gene="EGFP reporter"
ORF 3096..3815
/sequence="ORF_3 rf(3)"
misc_binding 3817..3824
/dbxref="REBASE:NotI"
misc_binding 3824..3829
/dbxref="REBASE:XhoI"
Tag 3830..3847
/gene="6xHis tag"
Regulatory_Seq 3863..4099
/gene="SV40ER reg"
Promoter 3886..4154
/gene="SV40 prom"
Rep_Origin 4053..4130
/gene="SV40 origin"
misc_binding 4133..4145
/dbxref="REBASE:SfiI"
misc_binding 4184..4189
/dbxref="REBASE:StuI"
ORF 4284..5078
/sequence="ORF_4 rf(3)"
Marker 4287..5075
/gene="NTP_II marker"
misc_binding 5096..5101
/dbxref="REBASE:EcoRV"
Other Gene 5172..5224
/gene="delta_U3 other"
Other Gene 5225..5405
/gene="HIV-1_5_LTR other"
Rep_Origin 6111..6730
/gene="pBR322 origin"
Promoter 7383..7432
/gene="NEOKAN prom"
ORF 7521..8315
/sequence="ORF_5 rf(3)"
Marker 7524..8312
/gene="NTP_II marker"
Rep_Origin 8420..8725
/gene="f1 origin"
BASE COUNT 2231 a 2151 c 2432 g 1985 t 0 others