pLenti-III-HA
| Vector Name | pLenti-III-HA |
| VectorType | Lentiviral vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga
2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag
2581 actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt
2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2701 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2761 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
2821 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
2881 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
2941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta
3001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac
3061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
3121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
3181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
3241 tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag
3301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
3361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
3421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
3481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
3541 acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
3721 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
3781 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
3841 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
3901 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
3961 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
4021 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
4081 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
4141 ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta
4201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
4261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
4321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
4381 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
4441 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
4561 tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa
4621 atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa
4681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
4741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
5041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
5101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
5161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
5221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
5281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
5341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5521 tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa
5581 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc
5641 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
5701 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
5761 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
5821 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa
5881 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
5941 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
6001 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
6061 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
6121 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
6181 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
6241 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
6301 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
6361 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
6421 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
6481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
6541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
6601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
6661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta
6721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
6781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
6841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
6901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
6961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
7021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
7081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
7141 aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca
7201 atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg
7261 acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg
7321 atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc
7381 gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt
7441 cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg
7501 taatactctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
7561 tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg
7621 aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
7681 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
7741 gat
LOCUS dna 7743 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
misc_binding 2462..2467
/dbxref="REBASE:SmaI"
misc_binding 2462..2467
/dbxref="REBASE:XmaI"
misc_binding 2468..2473
/dbxref="REBASE:ClaI"
misc_binding 2492..2497
/dbxref="REBASE:KpnI"
misc_binding 2519..2524
/dbxref="REBASE:EcoRI"
misc_binding 2537..2542
/dbxref="REBASE:StuI"
misc_binding 2543..2548
/dbxref="REBASE:HincII"
misc_binding 2549..2554
/dbxref="REBASE:EcoRV"
misc_binding 2555..2560
/dbxref="REBASE:ApaI"
misc_binding 2561..2568
/dbxref="REBASE:NotI"
misc_binding 2570..2575
/dbxref="REBASE:BamHI"
misc_binding 2576..2581
/dbxref="REBASE:XbaI"
misc_binding 2588..2593
/dbxref="REBASE:XhoI"
Promoter 2638..2906
/gene="SV40 prom"
Rep_Origin 2805..2882
/gene="SV40 origin"
ORF 2872..3540
/sequence="ORF_2 rf(1)"
misc_binding 2885..2897
/dbxref="REBASE:SfiI"
Marker 2941..3540
/gene="puro marker"
Other Gene 4150..4202
/gene="delta_U3 other"
Other Gene 4203..4383
/gene="HIV-1_5_LTR other"
Rep_Origin 4890..5509
/gene="pBR322 origin"
Promoter 5767..5816
/gene="NEOKAN prom"
misc_binding 5874..5879
/dbxref="REBASE:BclI"
ORF 5905..6699
/sequence="ORF_3 rf(1)"
Marker 5908..6696
/gene="NTP_II marker"
Rep_Origin 6804..7110
/gene="f1 origin"
Other Gene 7165..7266
/gene="RITR other"
Other Gene 7165..7266
/gene="LITR other"
Other Gene 7346..7494
/gene="Encap other"
BASE COUNT 1937 a 1906 c 2120 g 1780 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga
2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag
2581 actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa cacgcgtggt
2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2701 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2761 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
2821 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
2881 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
2941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta
3001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt ggacccggac
3061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
3121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
3181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
3241 tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg gcccaaggag
3301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
3361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
3421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
3481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
3541 acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
3721 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
3781 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
3841 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
3901 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
3961 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
4021 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
4081 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
4141 ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta
4201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
4261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
4321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
4381 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
4441 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
4561 tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa
4621 atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa
4681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
4741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
5041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
5101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
5161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
5221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
5281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
5341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5521 tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa
5581 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc
5641 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
5701 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
5761 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
5821 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa
5881 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
5941 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
6001 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
6061 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
6121 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
6181 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
6241 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
6301 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
6361 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
6421 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
6481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
6541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
6601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
6661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta
6721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
6781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
6841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
6901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
6961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
7021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
7081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
7141 aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca
7201 atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg
7261 acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg
7321 atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc
7381 gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt
7441 cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg
7501 taatactctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
7561 tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg
7621 aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
7681 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
7741 gat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC
1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920
GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT
1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980
CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG
NdeI
|
1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040
GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG
2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100
CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC
2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160
GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT
2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220
TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT
CMV prom(2279,2360)>>>
|
2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280
TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC
2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340
GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG
2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400
CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT
tetO reg(2416,2455)>>>
|
2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460
CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC
SmaI
|
XmaI ClaI KpnI
| | | |
2461 ccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgcaggtga 2520
GGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACGTCCACT
EcoRV NotI
| |
EcoRI StuI HincII ApaI BamHI XbaI
| | | | | | | |
2521 attcactagtaccggtaggcctgtcgacgatatcgggcccgcggccgctggatcctctag 2580
TAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATC
XhoI SV40 prom(2638,2906)>>>
| |
2581 actgcagctcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggt 2640
TGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCA
2641 gtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagt 2700
CACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCA
2701 cagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgc 2760
GTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACG
SV40 origin(2805,2882)>>>
|
2761 atctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactc 2820
TAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAG
ORF_2 rf(1)(2872,3540)>>>
|
2821 cgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagagg 2880
GCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCC
SfiI
|
2881 ccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcc 2940
GGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGG
puro marker(2941,3540)>>>
|
2941 atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgta 3000
TACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCAT
3001 cgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggac 3060
GCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTG
3061 cgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgac 3120
GCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTG
3121 atcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggag 3180
TAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTC
3181 agcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggt 3240
TCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCA
3241 tcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggag 3300
AGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTC
3301 cccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggc 3360
GGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCG
3361 agcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctg 3420
TCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGAC
3421 gagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgcc 3480
CTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGG
3481 gacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctga 3540
CTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACT
3541 acgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattctt 3600
TGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAA
3601 aactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgct 3660
TTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGA
3661 attgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctt 3720
TAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAA
3721 tatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgac 3780
ATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTG
3781 gcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgct 3840
CGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGA
3841 ttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggaca 3900
AAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGT
3901 ggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtccttt 3960
CCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAA
3961 ccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtc 4020
GGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAG
4021 ccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcct 4080
GGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGA
4081 cttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgc 4140
GAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCG
delta_U3 other(4150,4202)>>>
|
4141 ctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgta 4200
GACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACAT
HIV-1_5_LTR other(4203,4383)>>>
|
4201 ctgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacc 4260
GACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGG
4261 cactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgt 4320
GTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACA
4321 tgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctcta 4380
ACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGAT
4381 gcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatat 4440
CGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATA
4441 cagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagca 4500
GTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGT
4501 tcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaac 4560
AGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTG
4561 tcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaa 4620
AGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTT
4621 atggcattatgggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaa 4680
TACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTT
4681 ataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc 4740
TATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAG
4741 actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg 4800
TGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGC
4801 gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc 4860
CATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCG
pBR322 origin(4890,5509)<<<
|
4861 cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc 4920
GTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCG
4921 ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga 4980
GGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCT
4981 ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc 5040
GATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGG
5041 ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat 5100
GACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTA
5101 agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg 5160
TCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACAC
5161 cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc 5220
GTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGG
5221 aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga 5280
TTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCT
5281 gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact 5340
CGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGA
5341 agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt 5400
TCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAA
5401 ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag 5460
CCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTC
5461 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg 5520
GTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCC
5521 tctgacgctcagtggaacgaaaactcacgttaagggattttggccatgagattatcaaaa 5580
AGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTT
5581 aggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccc 5640
TCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGG
5641 cggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaag 5700
GCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTC
5701 caggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagca 5760
GTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGT
NEOKAN prom(5767,5816)>>>
|
5761 agcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagta 5820
TCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCAT
BclI
|
5821 aactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaa 5880
TTGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTT
NTP_II marker(5908,6696)>>>
|
ORF_3 rf(1)(5905,6699)>>>
| |
5881 gagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccg 5940
CTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGC
5941 gccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctct 6000
CGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGA
6001 gatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgac 6060
CTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTG
6061 ctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacg 6120
GACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGC
6121 acgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctg 6180
TGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGAC
6181 ctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaa 6240
GATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTT
6241 gtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgccca 6300
CATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGT
6301 ttcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtctt 6360
AAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAA
6361 gtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcc 6420
CAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGG
6421 aggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgc 6480
TCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACG
6481 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctg 6540
AACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGAC
6541 ggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagctt 6600
CCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAA
6601 ggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcag 6660
CCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTC
6661 cgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgtta 6720
GCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAAT
6721 aatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaaga 6780
TTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCT
f1 origin(6804,7110)<<<
|
6781 atagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaa 6840
TATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTT
6841 cgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtga 6900
GCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACT
6901 accatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccc 6960
TGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGG
6961 taaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagga 7020
ATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCT
7021 agggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcg 7080
TCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGC
7081 cgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattc 7140
GCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAG
LITR other(7165,7266)>>>
|
RITR other(7165,7266)<<<
|
7141 aggatcgaattaattcttaattaacatcatcaataatataccttttttggattgaagcca 7200
TCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGT
7201 atatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtg 7260
TATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCAC
7261 acgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacgg 7320
TGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCC
Encap other(7346,7494)>>>
|
7321 atgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgc 7380
TACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCG
7381 gcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccatttt 7440
CGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAA
7441 cgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcg 7500
GCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGC
7501 taatactctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggt 7560
ATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCA
7561 tccgcgcacatttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcg 7620
AGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGC
7621 aaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaa 7680
TTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTT
7681 catgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtac 7740
GTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATG
7741 gat 7743
CTA
LOCUS dna 7743 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
misc_binding 2462..2467
/dbxref="REBASE:SmaI"
misc_binding 2462..2467
/dbxref="REBASE:XmaI"
misc_binding 2468..2473
/dbxref="REBASE:ClaI"
misc_binding 2492..2497
/dbxref="REBASE:KpnI"
misc_binding 2519..2524
/dbxref="REBASE:EcoRI"
misc_binding 2537..2542
/dbxref="REBASE:StuI"
misc_binding 2543..2548
/dbxref="REBASE:HincII"
misc_binding 2549..2554
/dbxref="REBASE:EcoRV"
misc_binding 2555..2560
/dbxref="REBASE:ApaI"
misc_binding 2561..2568
/dbxref="REBASE:NotI"
misc_binding 2570..2575
/dbxref="REBASE:BamHI"
misc_binding 2576..2581
/dbxref="REBASE:XbaI"
misc_binding 2588..2593
/dbxref="REBASE:XhoI"
Promoter 2638..2906
/gene="SV40 prom"
Rep_Origin 2805..2882
/gene="SV40 origin"
ORF 2872..3540
/sequence="ORF_2 rf(1)"
misc_binding 2885..2897
/dbxref="REBASE:SfiI"
Marker 2941..3540
/gene="puro marker"
Other Gene 4150..4202
/gene="delta_U3 other"
Other Gene 4203..4383
/gene="HIV-1_5_LTR other"
Rep_Origin 4890..5509
/gene="pBR322 origin"
Promoter 5767..5816
/gene="NEOKAN prom"
misc_binding 5874..5879
/dbxref="REBASE:BclI"
ORF 5905..6699
/sequence="ORF_3 rf(1)"
Marker 5908..6696
/gene="NTP_II marker"
Rep_Origin 6804..7110
/gene="f1 origin"
Other Gene 7165..7266
/gene="RITR other"
Other Gene 7165..7266
/gene="LITR other"
Other Gene 7346..7494
/gene="Encap other"
BASE COUNT 1937 a 1906 c 2120 g 1780 t 0 others