pLenti-III-PGK
| Vector Name | pLenti-III-PGK |
| VectorType | Lentiviral vector |
| Antibiotic Information | Kanamycin in Bacterial, Puromycin in Mammalian |
| Sequencing Primers | PGK Sequencing Primer 5'---AAT TCT ACC GGG TAG GGG AGG CGC T---3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagaaatt ctaccgggta ggggaggcgc ttttcccaag
1861 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
1921 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
1981 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
2041 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
2101 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
2161 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
2221 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
2281 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctc
2341 aattgagtac ttacgtaggt accccagtgt ggtggcctgc aggtgaattc actagtaccg
2401 gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc ctctagactg cagctcgagt
2461 acccatacga cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca
2521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2581 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2641 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
2701 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
2761 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa
2821 gcccacggtg cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc
2881 cgcgttcgcc gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg
2941 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
3001 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
3061 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
3121 gcagcaacag atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
3181 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
3241 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
3301 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
3361 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa
3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
3541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
3601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
3661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
3781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
3841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
3901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc
3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa
4021 gggctaattc actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg
4081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
4141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
4201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
4261 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
4441 tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt
4501 attatgggtc tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg
4561 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
4621 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
4681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
4741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
4801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
4861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
4921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
4981 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
5041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
5101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
5161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
5221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
5281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
5341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
5401 gaacgaaaac tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta
5461 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
5521 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
5581 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
5641 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
5701 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg
5761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
5821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
5881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
5941 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
6001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
6061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
6121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
6181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
6241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
6301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
6361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
6421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
6481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
6541 tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt
6601 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
6661 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
6721 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
6781 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
6841 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
6901 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
6961 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat
7021 tcttaattaa catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg
7081 gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg
7141 cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg
7201 acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg
7261 atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga
7321 ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga
7381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
7441 cccgaaaagt gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta
7501 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
7561 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgat
LOCUS dna 7609 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2358..2363
/dbxref="REBASE:KpnI"
misc_binding 2385..2390
/dbxref="REBASE:EcoRI"
misc_binding 2409..2414
/dbxref="REBASE:HincII"
misc_binding 2415..2420
/dbxref="REBASE:EcoRV"
misc_binding 2421..2426
/dbxref="REBASE:ApaI"
misc_binding 2427..2434
/dbxref="REBASE:NotI"
misc_binding 2436..2441
/dbxref="REBASE:BamHI"
misc_binding 2442..2447
/dbxref="REBASE:XbaI"
misc_binding 2454..2459
/dbxref="REBASE:XhoI"
Promoter 2504..2772
/gene="SV40 prom"
Rep_Origin 2671..2748
/gene="SV40 origin"
ORF 2738..3406
/sequence="ORF_2 rf(2)"
misc_binding 2751..2763
/dbxref="REBASE:SfiI"
Marker 2807..3406
/gene="puro marker"
Other Gene 4016..4068
/gene="delta_U3 other"
Other Gene 4069..4249
/gene="HIV-1_5_LTR other"
Rep_Origin 4756..5375
/gene="pBR322 origin"
Promoter 5633..5682
/gene="NEOKAN prom"
misc_binding 5740..5745
/dbxref="REBASE:BclI"
ORF 5771..6565
/sequence="ORF_3 rf(2)"
Marker 5774..6562
/gene="NTP_II marker"
Rep_Origin 6670..6976
/gene="f1 origin"
Other Gene 7031..7132
/gene="RITR other"
Other Gene 7031..7132
/gene="LITR other"
Other Gene 7212..7360
/gene="Encap other"
BASE COUNT 1857 a 1909 c 2124 g 1719 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagaaatt ctaccgggta ggggaggcgc ttttcccaag
1861 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
1921 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
1981 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
2041 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
2101 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
2161 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
2221 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
2281 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctc
2341 aattgagtac ttacgtaggt accccagtgt ggtggcctgc aggtgaattc actagtaccg
2401 gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc ctctagactg cagctcgagt
2461 acccatacga cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca
2521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2581 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2641 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
2701 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
2761 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa
2821 gcccacggtg cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc
2881 cgcgttcgcc gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg
2941 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
3001 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
3061 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
3121 gcagcaacag atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
3181 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
3241 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
3301 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
3361 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa
3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
3541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
3601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
3661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
3781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
3841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
3901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc
3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa
4021 gggctaattc actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg
4081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
4141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
4201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
4261 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
4441 tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt
4501 attatgggtc tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg
4561 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
4621 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
4681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
4741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
4801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
4861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
4921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
4981 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
5041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
5101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
5161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
5221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
5281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
5341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
5401 gaacgaaaac tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta
5461 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
5521 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
5581 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
5641 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
5701 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg
5761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
5821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
5881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
5941 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
6001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
6061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
6121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
6181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
6241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
6301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
6361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
6421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
6481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
6541 tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt
6601 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
6661 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
6721 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
6781 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
6841 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
6901 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
6961 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat
7021 tcttaattaa catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg
7081 gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg
7141 cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg
7201 acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg
7261 atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga
7321 ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga
7381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
7441 cccgaaaagt gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta
7501 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
7561 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
1801 aaattttcgggtttttcgaacctagaaattctaccgggtaggggaggcgcttttcccaag 1860
TTTAAAAGCCCAAAAAGCTTGGATCTTTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTC
1861 gcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcct 1920
CGTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGA
1921 ctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtgg 1980
GACCGGAGCGTGTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACC
1981 ccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagct 2040
GGGGAAGCGCGGTGGAAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGA
2041 cgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatgga 2100
GCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCT
2101 cagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttg 2160
GTCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAAC
2161 ctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcagggg 2220
GAGGAAGCGAAAGACCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCC
2221 cgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgct 2280
GCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGA
2281 tcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctc 2340
AGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCTGGAG
KpnI EcoRI
| |
2341 aattgagtacttacgtaggtaccccagtgtggtggcctgcaggtgaattcactagtaccg 2400
TTAACTCATGAATGCATCCATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGC
EcoRV NotI
| |
HincII ApaI BamHI XbaI XhoI
| | | | | | |
2401 gtaggcctgtcgacgatatcgggcccgcggccgctggatcctctagactgcagctcgagt 2460
CATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCA
SV40 prom(2504,2772)>>>
|
2461 acccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtcccca 2520
TGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGT
2521 ggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgt 2580
CCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACA
2581 ggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtca 2640
CCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGT
SV40 origin(2671,2748)>>>
|
2641 gcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcc 2700
CGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGG
SfiI
|
ORF_2 rf(2)(2738,3406)>>>
| |
2701 cattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcg 2760
GTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGC
puro marker(2807,3406)>>>
|
2761 gcctctgagctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaa 2820
CGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTT
2821 gcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgc 2880
CGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCG
2881 cgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcg 2940
GCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGC
2941 ggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtg 3000
CCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACAC
3001 ggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcggg 3060
CCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCC
3061 ggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgc 3120
CCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCG
3121 gcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcct 3180
CGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGA
3181 ggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgct 3240
CCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGA
3241 ccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgcc 3300
GGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGG
3301 ccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcc 3360
GGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGG
3361 cgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaa 3420
GCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTT
3421 tcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctcc 3480
AGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGG
3481 ttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtat 3540
AAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATA
3541 ggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtg 3600
CCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACAC
3601 gcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactgg 3660
CGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACC
3661 ttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctat 3720
AACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATA
3721 tgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgtt 3780
ACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAA
3781 gggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgc 3840
CCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCG
3841 ctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaa 3900
GACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTT
3901 tccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgc 3960
AGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCG
delta_U3 other(4016,4068)>>>
|
3961 cttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaa 4020
GAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTT
HIV-1_5_LTR other(4069,4249)>>>
|
4021 gggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctgg 4080
CCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACC
4081 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 4140
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
4141 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 4200
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
4201 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttca 4260
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGT
4261 tgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagagg 4320
ACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCC
4321 aacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcaca 4380
TTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGT
4381 aataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatct 4440
TTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGA
4441 tatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggt 4500
ATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCA
4501 attatgggtctgcattaatgaatcggccaacggatgtcggtgtgaaataccgcacagatg 4560
TAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTAC
4561 cgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcg 4620
GCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGC
4621 ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc 4680
GAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAG
4681 cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccag 4740
GTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTC
pBR322 origin(4756,5375)<<<
|
4741 gaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagca 4800
CTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGT
4801 tcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacca 4860
AGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGT
4861 ggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg 4920
CCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCC
4921 atacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtag 4980
TATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATC
4981 gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgt 5040
CATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCA
5041 tcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagaca 5100
AGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGT
5101 cgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtagg 5160
GCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCC
5161 cggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatt 5220
GCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAA
5221 tggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc 5280
ACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAG
5281 cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcg 5340
GCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGC
5341 cagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtg 5400
GTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCAC
5401 gaacgaaaactcacgttaagggattttggccatgagattatcaaaaaggatcttcaccta 5460
CTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGAT
5461 gatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcag 5520
CTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTC
5521 ctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcag 5580
GATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTC
NEOKAN prom(5633,5682)>>>
|
5581 tgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaatt 5640
ACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAA
5641 gccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggcttt 5700
CGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAA
BclI
|
5701 cttgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgagg 5760
GAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCC
NTP_II marker(5774,6562)>>>
|
ORF_3 rf(2)(5771,6565)>>>
| |
5761 atcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtgga 5820
TAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCT
5821 gaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgtt 5880
CTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAA
5881 ccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccct 5940
GGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGA
5941 gaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttg 6000
CTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAAC
6001 cgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagt 6060
GCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCA
6061 gccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggc 6120
CGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCG
6121 tgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagc 6180
ACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCG
6181 gaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatga 6240
CTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACT
6241 tctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgag 6300
AGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTC
6301 catgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcat 6360
GTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTA
6361 ggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccg 6420
CCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGC
6421 ctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggc 6480
GATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCG
6481 tgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttcta 6540
ACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGAT
6541 tcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcatttt 6600
AGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAA
6601 ttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatag 6660
AATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATC
f1 origin(6670,6976)<<<
|
6661 ggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacg 6720
CCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGC
6721 tcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaat 6780
AGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTA
6781 caagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagccccc 6840
GTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGG
6841 gatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcga 6900
CTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCT
6901 aaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacac 6960
TTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTG
6961 ccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaat 7020
GGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTA
RITR other(7031,7132)<<<
|
LITR other(7031,7132)>>>
|
7021 tcttaattaacatcatcaataatataccttttttggattgaagccaatatgataatgagg 7080
AGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCC
7081 gggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtgg 7140
CCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACC
7141 cggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtg 7200
GCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCAC
Encap other(7212,7360)>>>
|
7201 acgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcgg 7260
TGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCC
7261 atgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactga 7320
TACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACT
7321 ataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatactctcatga 7380
TATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACT
7381 gcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttc 7440
CGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAG
7441 cccgaaaagtgccacctgacgtaactataacggtcctaaggtagcgaaaatgtagtctta 7500
GGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAAT
7501 tgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaagg 7560
ACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCC
7561 agagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgat 7609
TCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 7609 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2358..2363
/dbxref="REBASE:KpnI"
misc_binding 2385..2390
/dbxref="REBASE:EcoRI"
misc_binding 2409..2414
/dbxref="REBASE:HincII"
misc_binding 2415..2420
/dbxref="REBASE:EcoRV"
misc_binding 2421..2426
/dbxref="REBASE:ApaI"
misc_binding 2427..2434
/dbxref="REBASE:NotI"
misc_binding 2436..2441
/dbxref="REBASE:BamHI"
misc_binding 2442..2447
/dbxref="REBASE:XbaI"
misc_binding 2454..2459
/dbxref="REBASE:XhoI"
Promoter 2504..2772
/gene="SV40 prom"
Rep_Origin 2671..2748
/gene="SV40 origin"
ORF 2738..3406
/sequence="ORF_2 rf(2)"
misc_binding 2751..2763
/dbxref="REBASE:SfiI"
Marker 2807..3406
/gene="puro marker"
Other Gene 4016..4068
/gene="delta_U3 other"
Other Gene 4069..4249
/gene="HIV-1_5_LTR other"
Rep_Origin 4756..5375
/gene="pBR322 origin"
Promoter 5633..5682
/gene="NEOKAN prom"
misc_binding 5740..5745
/dbxref="REBASE:BclI"
ORF 5771..6565
/sequence="ORF_3 rf(2)"
Marker 5774..6562
/gene="NTP_II marker"
Rep_Origin 6670..6976
/gene="f1 origin"
Other Gene 7031..7132
/gene="RITR other"
Other Gene 7031..7132
/gene="LITR other"
Other Gene 7212..7360
/gene="Encap other"
BASE COUNT 1857 a 1909 c 2124 g 1719 t 0 others