pLenti-III-Promoterless

                            FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2904..2930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      3115..3250
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3251..3850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    3863..4450
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4334..4345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4460..4693
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4513..4693
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    4765..4886
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5215..5803)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6215..7009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga
     1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt
     1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac
     2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     2161 cagcagcgac cgcccggggc tccgctccgg tccggcgctc cccccgcatc ccgagccggc
     2221 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
     2281 ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaga attgggggag
     2341 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     2401 gggggcagca gcgaccgccc ggggctccgc tccggtccgg cgctcccccc catcccgagc
     2461 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc ttcctctgaa
     2521 cgcttctcgc tgctctttga gcctgcagac acctggggga tacggggaaa aacactgggg
     2581 tacctacgta agggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta
     2641 cgtccctccc cgctaggggg cagcagcgac cgcccggggc tccgctccgg tccggcgctc
     2701 cccccgcatc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     2761 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tgggggatac
     2821 ggggaaaaga attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg
     2881 gatcctctag actgcagctc gagtacccat acgacgtccc agactacgct tgagtttaaa
     2941 cacgcgtggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3241 tttggaggcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3301 tcgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3361 ggacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3421 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     3481 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     3541 gttgagcggt tcccggctgg ccgcgcagca acagatggaa gggctcctgg cgccgcaccg
     3601 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     3661 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     3721 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     3781 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     3841 cggtgcctga acgcgttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac
     3901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4381 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4441 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt
     4501 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4681 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4741 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4861 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag
     4921 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacggatg
     4981 tcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggccatgag
     5881 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
     5941 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     6001 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     6061 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     6121 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     6181 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6241 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6301 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6361 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6421 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6481 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6541 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6601 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6661 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6721 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6781 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6841 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6901 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6961 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa
     7021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     7441 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata ccttttttgg
     7501 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac
     7561 ggggcgggtg acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg
     7621 taagcgacgg atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga
     7681 caattttcgc gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt
     7741 tggccatttt cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac
     7801 tcatagcgcg taatactctc atgagcggat acatatttga atgtatttag aaaaataaac
     7861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtaact ataacggtcc
     7921 taaggtagcg aaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7981 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8041 aaggtggtac gat
                        

                            FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2904..2930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      3115..3250
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3251..3850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    3863..4450
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4334..4345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4460..4693
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4513..4693
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    4765..4886
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5215..5803)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6215..7009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN