Site Directed Mutagenesis Service
abm has over 10 years’ experience with Site-Directed Mutagenesis Services and we have successfully completed hundreds of orders. Past projects include modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition/removal, base substitution, sequence deletion/insertion and promoter insertion/swapping. For large plasmids, large fragment insertions/deletions, difficult templates, and other mutagenesis requests, please inquire.
| Service | |
|---|---|
| Procedure | PCR-Based Mutagenesis |
| Delivery Format | Plasmid DNA |
| Lead Time | In as little as 2-3 weeks for orders of 1.0 μg of DNA |
| Quality Control | Full-length sequencing to confirm presence of mutation |
| DNA Submission Guidelines | At least 10μg DNA per mutated clone required (200ng/μl or higher). Plasmids must be high copy plasmids. For medium or low copy plasmids, please inquire. |
Additional Resources:
"Our lab has used abm's site-directed mutagenesis service many times, and you always produce great plasmids!"
Stuart Jarrett, University of KentuckyService Details
Core Services
| Service Name | Unit | Cat. No. | PRICE |
|---|---|---|---|
| Site-Directed Mutagenesis Service | Single Clone | C047 | $150.00 |
Additional Services
| Service Name | Unit | Cat. No. | Unit Price |
|---|---|---|---|
| DNA Sequencing | Single Reaction | C199 | $7.50 |
| DNA Sequencing with Custom Primer Synthesis | Single Reaction | C197 | $13.50 |
| Bacterial Glycerol Stock | 1 clone | C139 | $25.00 |
| Plasmid Amplification (10-20 μg) | 10‑20 μg | C309 | $75.00/prep |
| Plasmid Amplification (80-100 μg) | 80‑100 μg | C310 | $150.00/prep |
Mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector.
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).
Additional Info
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FAQs
| Does this also apply for insertions/deletions/mutations that span >25 consecutive base pairs? |
|
Pricing for Cat# C047 only applies for insertions/deletions/mutations that span <25 consecutive base pairs or fewer. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.
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Citations
| 01 | Wen, Z et al. "MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V." PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector. |
| 02 | Laryea, G et al. "Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors." Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector. |
| 03 | Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction. |
| 04 | Eagleman, DE et al." Unbiased proteomic screening identifies a novel role for the E3 ubiquitin ligase Nedd4-2 in translational suppression during ER stress." J Neurochem 157(6):1809-1820 (2021). DOI: 10.1111/jnc.15219 PubMed: 33064840 Application: Site Directed Mutagenesis. |